1) What role does the active site lysine play in the mechanism of CO2 fixation in Rubisco? Hypothesize what effect the mutation of this lysine to arginine would have on the protein.
Rubisco is a very crucial enzyme in carbon fixation and is an important protein in the living ecosystem. The active site Lysin acts as a catalyst in primary photosynthetic CO2 reduction reaction in Rubisco. Rubisco is in itself a weak catalyst for this reduction reaction. Also, it is prone to side reactions such as oxygenation of sugar phosphate by atmospheric oxygen.
The mutation of Lysine to Arginine again decreases the catalytic property as the specificity factor for CO2 in Rubisco and the rate of carboxylation reaction decreases.
1) What role does the active site lysine play in the mechanism of CO2 fixation in Rubisco? Hypoth...
What is required for the following reaction? CO2 # # O an active site lysine residue thiamine pyrophosphate as a coenzyme a Schiff base as an intermediate NAD as an oxidizing agent biotin as a coenzyme by causing the phosphorylation of Glucagon activates glycolysis. a regulatory enzyme of gluconeogenesis; hexokinase O gluconeogenesis: phosphofructokinase-1 gluconeogenesis, pyruvate kinase glycoslysis: phosphofructokinase-1 O glycoslysis: pyruvate kinase
The second part of the mechanism for histidyl-tRNA-synthetase is shown below. Active site residues are in black and the substrates are shown in green. Examine the image and complete the following questions. RNAH A76 Arg113 Gin 127 Glu131 M-o Histidy-Adenylate Arg259 Tyr264 c 1. Describe the role of Arg259 in the active site of histidyl- RNA-synthetase. Proton anor acid 2. If the substrates for this enzyme are histidine, ATP, and tRNAHs. Describe overall (not mechanism steps) what must have happened...
90% of cancer cells have active telomerase enzymes. How does this play a role in cancer and how they divide fast.
What is the effect of hydrogen peroxide on e.coli and what role does oxidative stress play in this?
1) histone proteins have a high concentration of the amino acids Lysine and Arginine. what property makes them ideal for DNA attraction? 2) T-loops are protected from degradation by deoxyribonucleases because of the telomere-specific protein complex called ____?
The following questions are based on the papin mechanism below: Explain the role of each amino acid in the catalytic triad. Site directed mutagenesis replacing the active site asp in the papain protease with an ala showed that catalytic activity was not significantly affected. Why would you think that asp is not so critical in the cysteine proteases? Hi R CH2 NH-R oe 0 hole oxyanion hole 0 oxyanion hole CH a) Hi R CH2 NH-R oe 0 hole oxyanion...
What role does current information management technology play in preparing nurses for clinical practice in the twenty-first century? Explain why. What effect does future healthcare delivery have on vulnerable populations? Include at least two examples from the field of nursing? Specifically maternal health?
Describe play in early childhood. What role does play have in socioemotional development during this stage of development? Grammar and spelling does matter. Your answer should be 4 paragraphs in length.
What role does mRNA play in the process of protein synthesis? Choose all that apply. Select one or more: Every three nucleotides binds an amino acid It opens up the DNA strand for transcription to occur ] c. It is a template for the arrangement of tRNAS 1 d. It determines the order in which amino acids are added to the growing protein e. It copies DNA to be used in later steps
What role does control of gene expression play in the diversity of individuals of the same species within a population, and how does this individual diversity contribute to natural selection? Point mutations within the DNA are often considered neutral, in that they have no discernable effect on the phenotype of the affected individual. Why is this the case? What properties of the genetic code contribute to the neutrality of point mutations? Why are mutations that add or subtract nucleotides within...