The following questions are based on the papin mechanism below: Explain the role of each amino acid in the catalytic triad. Site directed mutagenesis replacing the active site asp in the papain protea...

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The following questions are based on the papin mechanism below:

Explain the role of each amino acid in the catalytic triad.

Site directed mutagenesis replacing the active site asp in the papain protease with an ala showed that catalytic activity was not significantly affected. Why would you think that asp is not so critical in the cysteine proteases?

Hi R CH2 NH-R oe 0 hole oxyanion hole 0 oxyanion hole CH a)

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Answer 1

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でAntal se bucd consisting Cys 25, His is dine 1s i abilized ipmidlazole im he sub srab Aan cay anion hole, famed io the nilog These mesidues ina ct thein interface Av cipain Pheop has shounm inabilitu to undwit activation at its optimum stesidue. help

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The following questions are based on the papin mechanism below: Explain the role of each amino acid in the catalytic triad. Site directed mutagenesis replacing the active site asp in the papain protea...

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The following questions are based on the papin mechanism below: Explain the role of each amino acid in the catalytic triad. Site directed mutagenesis replacing the active site asp in the papain protea...

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