Question

5. Based on your understanding of the mechanism of proteases A. the figure below shows a part of the active site of the enzyme chymotrypsin. what are the identities of amino acids x and Y and what is this particular type of spatial arrangement of amino acids called? Alkoxide on X ? B. from the strucutres shown below, identify the two that represent the first and the second tetrahedral intermediates in the chymotrypsin catalyzed reaction. what type of attack and by which molecules result in the formation of the first and the sencond intermediates, respectively? ycspecuvery? (4 poi a. b. C. d. O R C NH-Enz R R CNH R C NH Enz R-C R-C-O-Enz O Enz OH OH Enz C. based on the structure you identified as the first tetrahedral intermediate of chymotrypsin's substrate cleavage reaction, draw a simple chemical structure (like above) of what the first intermediate would look like for a cysteine protease D. attack by which molecule/group results in the formation of the first tetrahedral intermediate in aspartyl proteases and how is this molecule/group activated?

Answer 1

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