Question

l Review Constants | Periodic Table Assume that a FRET donor-acceptor pair is composed of the fluorescent dyes Alexa Fluor 488 (excited-state lifetime of 4.1 ns) and Alexa Fluor 594. For this FRET pair Ro 5.4 nm The distance between the FRET pair ranges from 2.0 nm to 12.5 nm for the biomolecule of interest. Calculate the energy-transfer rate for r = 1.0 nm Express your answer in reciprocal seconds to two significant figures. kret - Submit Calculate the energy-transfer rate for r 6.9 nm Express your answer in reciprocal seconds to two significant figures kret Submit Part C Calculate the energy-transfer rate for r 10.3 nm

Answer 1

The energy transfer rate =k Excited state lifetime TD donor-acceptor distance=r =4.1 ns = 4.1×10-9 s R, 5.4 nm 9 nm The energy transfer rate =kr,-- - The energy transfer rate-kret- The energy transfer rate k-1.3x10" s nm er41x109 s 1.9 nm ret

Part - B r-6.9 nm The energy transfer rate ky The energy transfer rate ky - The energy transfer rate 5.6x10 s1 Part- C 1 CR ret 5.4 nm rer4.1x10 s 6.9 nm r-10.3 nnm The energy transfer rate =kretーー The energy transfer rate-kret- The energy transfer rate= kret-5.1x10° s-I 1 (R 5.4 nm 4.1x10 10.3 m