Your lab assistant put together DNA sequences to create a modified polypeptide sequence, then exp...
your lab assistant put together DNA sequences to create a modified polypeptide sequence, then expressed these constructs in some brain cells. For each of the following constructs, predict the intracellular (or extracellular) location, and a brief description of why. Assume a start codon is present.
a) a protein normally targetted to the chloroplast stroma, with N-terminal targeting sequence deleted.
b) a C-terminal peroxisomal targeting sequence has been asses to the C-terminus of a normally secreted protein, still with its usual N-terminal signal sequence.
c) the addition of an N-terminal mitochondrial targeting sequence to a protein with internal nuclear localization sequences.
d) moving the N-terminal signal sequence of a secreted protein to the C-terminus of the protein.
I am very lost so please explain in some detail and/or if you know any videos or websites on these concepts I would be very interested! thanks!
Homework Answers
a. if the N-terminal targeting sequence is deleted, the protein will no more be recognized to be targeted to the chloroplast stroma. It will not go to chloroplast stroma. It will also not be secreted extracellularly. It may get stuck in the cytoplasm or chloroplast membrane.
b. the protein will still be recognized for secretion because its N-terminal signal sequence is intact. The peroxisomal target sequence is after the protein sequence which will not be prioritized by the cell.
c. the protein will now be localized to the mitochondria
d. it will remain intracellularly now, and won’t be secreted out of the cell
Add Answer to:
Your lab assistant put together DNA sequences to create a modified polypeptide sequence, then exp...
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sequence from the fragments. However, you do not need to write out
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1. Where is the ER targeting sequence located in the polypeptide chain (N-terminal, interior, or C-terminal) 2. Name 4 organelles that require organelle specific targeting sequences in polypeptide 3. Name 3 final locations for proteins with ER signal sequence 4. Satranslational translocation: (a) Name the protein that the signal sequence binds to (b) Name the protein SRP binds to 5. State the function of signal peptidase 6. Name a membrane protein that is not a single pass membrane protein 7....
want to double check!
25. Th e DNA sequences encoding the initiation whese parated bcoding the initiation and termination codons of a certain protein amino acids in length. there of the protei nucleotides on a certain organism's chromosome; however ssuming that there has been no post-translational processing elined from this gene is translated, the protein product is only 250 A. The RNA was synthesized in a bacterial cell n, what can you conclude from these observations? The RNA was synthesized...
6. Islet amyloid polypeptide (IAPP), often called amylin, is a peptide hormone that is co-secreted with insulin from pancreatic B-cells. In combination, insulin and amylin regulate blood glucose levels. In this signaling process, amylin participates in slowing gastric emptying and promoting satiety, helping to prevent spikes in blood glucose levels. Using your knowledge of protein sequencing and proteomics, answer the questions below related to amylin. A. Islet amyloid polypeptide (IAPP, amylin) is produced by processing of a precursor protein called...
Scientists want to design fluorescent marker proteins for different cellular compartments using the Green Fluorescent Protein (GFP). This means that the GFP will be targeted and glow at the organelle of interest and they can use these to mark different organelles during fluorescence microscopy. To make these marker proteins, they take the sequence of GFP and add the desired sorting signal. A. GFP on its own is an excellent protein to use as an organelle marker protein because it is...
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sequence from the fragments. However, you do not need to write out
the amino acid sequence - you can just provide the order of the
fragment numbers (from either set of fragments). For
example (and this is not the answer), you could say:
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