Question

(d) The authors state that protonated histidines were not observed to bind metal ions - binding to neutral histidines is an either/or process. In the absence of metal ions, the two histidines ligands each have pKa's of 6.5. What is the binding energy for these two protons together? -How do they compare with the binding energies for Zn2 and Co+ (calculate these as well)? -How were each of these binding constants determined? -If you determined the histidine pKa's in the presence of a small concentration, say 10 μΜ, of metal ion, which of the two ions would cause the effective pKa to be lowest?

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Answer 1

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Saluchcn HJ han (九

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