Question

Name l_ent 11 Lab Report-ENZYMES: HYDROLYSIS OF STARCH BY AMYLASE Partner Section l113 Station # PART B. Hydrolysis of starch by increasing amounts of extract Table 1. Absorbance at 590 nm after 5 min incubation of starcn % of starch remaining ps of | Absorbance at 590 | oextract nm after incubation after 5 min incubation 100 0 .312 laa 3 3 5 absorbance of tube O (no enzyme) reflects the total amount of starch initially nt, so the % starch remaining in each tube can be calculated by this equation: prese Absorbance after incubation x 100 % of starch remaining . Absorbance of tube rd the fewest # of drops of extract that results in 3590 or less of the 0 Reco starch remaining: drops of extract This is the number of drops of extract that you will add in Part C 1. What molecule is being detected by measuring the absorbance at 590 nm? 2. Why does the absorbance decrease as the amount of extract added increases? 3. How would you expect the absorbance of tube 1 to change (increase, decrease, or stay the same) if it was incubated for 10 minutes rather than for 5 minutes? Explain your prediction. Would you expect the absorbance of tube 0 to change following a 10-minute incubation? Explain. 4. 149

Answer 1

Ans 1. the molecule is being detected by measuring the absorbance at 590 is starch because the absorbance is decreasing after the incubation of the same with the extract.

Ans 2. The extract contains the enzyme amylase which will cleave the starch. As the volume of the extract increases, the amount of enzyme also will increase that will eventually degrade more starch. Hence there will be a drop in the absorbance at 590 nm

Ans 3. Certainly the absorbance at 590nm of the the 'tube 1' will decrease as the extract (enzyme) is added to it. The activity of the enzymes will be depending on the concentration and time.

Ans 4. There will not be any significant change in the absorbance of 'tube 0' even after 10 minutes as it doesn't contain the extract (enzyme).