Question

1. Extensive treatment with phospholipase A2 of a right-side-out, sealed plasma membrane vesicle preparation from a human fibroblast cell line hydrolyzes a fraction of the lipids in each phospholipid class (see table below). Note that phospholipase A2 hydrolyzes the ester linkage on the fatty acyl chain at the 2-position of a phosphoglyceride. Assuming that the accessible substrate lipids are present on both leaflets (monolayers) of the plasma membrane, calculate the phospholipid composition of each leaflet. Report the calculated percentage of each phospholipid composing the outer leaflet (these should sum to 100%) and of those composing the inner leaflet (these should also sum to 100%). % of the lipid in each class hydrolyzed by phospholipase A2 (PLA2) treatment % of total lipids in each class Lipid class 70 30 0 10 Phosphatidylcholine (PC) Phosphatidylethanolamine (PE) Sphingomyelin (SP) Phosphatidylinositol (Pl) Phosphatidylserine (PS) 40 30 15 10

Answer 1

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