Question

4. Genotype Results Were you successful in isolating genomic DNA and obtaining a PCR product? (Describe size and estimated amount.) If answering No to either, explain why! a. /) enimenon. TC alle )/.tet e t。 /1</i J What is your genotype as revealed by the Fnu4HI digest of your PCR product and if it matches your phenotype? b. c. Why might an individual's phenotype not match their genotype?

Answer 1

Answer a:)

Yes, we obtained the PCR product. The size of the product is 200bp.

Answer b:)

In the Fnu4H1 digestion, both bands are on same position. Therefore, these are homozygous. We cannot tell the genotype until we do not have their phenotypes.

"""""""""Please rate the answer""""""""""