Question

biochemistry glycolosis

Part 2: Glycolysis and Radiolabeling A common practice in biochemistry is the feeder study, in which radiolabeled substrate is fed to an organism; afterwards, radiolabeled metabolites are isolated and used to determine how the substrate was used by the organism. 4. Plants use intermediates from the glycolytic pathway as substrates for other pathways. For example, if we feed plants radiolabeled glucose, the radiolabel eventually ends up in the amino acid valine. If we feed the plants 6-(C)glucose i.e. carbon 6 of glucose is "C- where will the "C be in valine? To answer this question, write out the pathway for glycolysis. Mark it with a highlighter. Follow this carbon throughout the pathway and the carbons of pyruvate should bear the label. Next, take this labeled of valine shown below. Full credit is given to those who show the and valine biosynthesis. (20 pts) carbon 6 of glucose with an asterisk or highlight highlight it after each step. By the end, one of pyruvate through the biosynthetic pathway labeled carbon in each step of both of glycolysis

Answer 1

After fourth step of glycolysis, we further take only radioactive carbon in process.

catbon -destinati On A TP ADPtH 3 iDsC plopho OH ATP oT on h lel ん NPDM nas ATP ta pi cerale Cexa

nclase OA arbn mval kunan ATE COO CO, 부 tilata ale -cro ·ㄒㄧㄧ соо- aid hadis aehwe 13 kete LAO Va o valera valurale 贫。 branthd thain ale Cathon Valine