Question

Titaion of umino acid un give npu ph a) Can also ie yeo the buffer runye Two technes: protin assas, hbaton Isolation and characterization of plant pigment Ms. Cheron and Worrell isolated some pigment from broccoli during a biochemistry class. The pigment absorbances were obtained using a UV- Spectrophotometer. They obtained the following absorbance 3.782, 3.830 and 2.680 nm at 470, 662, and 645nm respectively. Calculated the concentration of chlorophyll a, b, total chlorophyll, ratio a. 70 6 of chlorophyll a/b and total carotenoids of the plant pigment. 645 b. Thin layer chromatography (TLC) was carried out to support the evidence that the pigments were carotenoids and chlorophyll. The distance travelled by the solvent cal pol from the origin was 5cm. Three components were observed to have a distance of ot sdvent,3cm, 2.1cm and 4.5cm using TLC. Calculate the retention factor of the components. What does the retention values of these components tells 0.1cm Hemo alobin has iron What is the difference between femoglobin and chlorophyls? Stte at least three differences between chlorophyll a and b. c. um. d. State at least (2) application of plant pigments in the following areas with &xpaniatron to support your answer 1. Pharmaceutical sciences 2. Medicine - Can be used to produce a more natrad med icin 3. Agriculture

Answer 1

b.

The retention factor = distance travelled by solute/distance travelled by solvent

So, Rf = 1.3/5 = 0.26

Rf = 2.1 / 5 = 0.42

Rf = 4.5/5 = 0.9

the 0.26 and 0.42 indicates polar compounds and 0.9 indicates non polar compounds

c.

Plant chlorophyll has magnesium in its structure

human haemoglobin has iron in its structure

Chlorophyll is green pigment

haemoglobin is a red pigment

chlorophyll involved in photosynthesis

haemoglobin involved in oxygen transportation

Chlorophyll is present in plants, cyanobacteria and algae

haemoglobin is present in human blood

d.

Pharmaceutical Sciences: The plant pigments are used as colouring agents or as a pharmaceutical aid.

Medicine: Carotenoids are used as antioxidants and flavonoids are used to lower cholesterol and as antioxidant