Question

18. In the active site of chymotrypsin, what is the major result of the hydrogen bonding of Histidine-57 to Serine-195? a) The pKa of the side chain of the serine is lowered b) It allows the formation of a tetrahedral intermediate between the histidine and the serine. c) It allows chymotrypsin to recognize the appropriate substrate. d) It prevents chymotrypsin from becoming active inside of the cell and allows it to become activated via proteolytic cleavage. It allows for proteolysis to be coupled to ATP hydrolysis resulting in a negative AG value. e)

Answer 1

Answer would be B

It allows formation of a tetrahedral intermediate between histidine and serine.

This occurs with the help of hydrogen bond and helps in stabilizing substrate in active site of enzymes and hence catalyse reaction

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