Question

Immunology

The immune system is self-restricted and the self-restriction is seen with immunological responses that involve both the MHC class I and the MHC class II molecules. Define MHC class I and class II restriction, which molecules and cells are involved and which stage of the immune response (stages 1 – 8 in figure 1-7 on page 20) the class I and class II restriction occurs (pick a stage from figure 1-7, state that stage, and describe the MHC restriction taking place (class I or II and the cells and molecules). Then describe an experiment which shows MHC class I restriction and then an experiment showing MHC class II restriction. Be sure to define, describe and name all antigens, animals, cell, purification systems and assays (readouts) used.

OVERVIEW FIGURE 1-7 Collaboration between Innate and Adaptive Immunity in Resolving an Infection Primary lymphoid organs Bone Lymphocyte marroW (Ch. 6, 7, 8, 9) Innate Epithelial cell barrier(Ch. 4, 5) Infection Blood flow IN lymphoid organ (e.g. lymph node) Lymphatic flow IN Lymphocyte Activation and Lymphatic flow OUTY Cut or splinter (Ch. 10, 11) 6 ImmunityAgents (Ch. 13) Memory Cells (Ch. 10, 11) (Ch. 17) Punt, Kuby Immunology, 8e, © 2018 W. H. Freeman and Company

Answer 1

• Definition of MHC class I and class II restriction and the molecules and cells are involved in these classes:

MHC restriction where the APC or target cells express MHC molecules that the T cell recognizes as self in order for T cell to respond to the antigen presented by that APC or target cell.

CD8 T cells bind class I MHC which are expressed on most cells in the body.

CD4 T cells bind class II MHC which are only expressed on specialized APCs.

• The stage of the immune response where the class I and class II restriction occurs: In the effector phase. In this phase of the cell-mediated response, activated macrophages carry out phagocytosis and cytolysis. Interruption of any of these steps results in significant cellular immune suppression.