Question

all parts please

3. Oxidative phosphorylation (50 pts) A) In his studies of alcoholic fermentation by yeast, Louis Pasteur noted that the sudden addition of oxygen (O,) to a previously anaerobic culture of fermenting grape juice resulted in a dramatic decrease in the rate of glucose consumption. This "Pasteur effect can be counteracted by the addition of 2.4-dinitrophenol (DNP), an uncoupler of oxidative phosphorylation. 0) Why would the yeast cells consume less glucose in the presence of oxygen? (ii) How much less glucose they would use? (ii) Why would DNP counteract or prevent the Pasteur effect? B) Which molecule has greater redox potential: NADH or FADH2? How is this difference in potential reflected in the flow of electrons into/through/out of the electron transport chain? What conditions/products/reagents specifically regulate oxidative phosphorylation C) (electron transport and chemiosmosis)? Explain. At which two steps of oxidative phosphorylation are protons "pumped" across the inner mitochondrial membrane? By what mechanism are other protons 'translocated across this membrane? D)

Answer 1

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