Question

Use the diagram below for questions 9-10. The black line indicates a locus, arrows indicate restriction sites and the red line indicates a probe to the region indicated. What does the arrow with the star/asterisk above it mean? Choose all possible answers. All of these answers are correct. None of these answers are correct. That restriction site is a SNP. That restriction site is a RFLP. That site will not vary between homologs within an individual. That site will not vary between individuals. Question 10 0.5 pts How many restriction fragments/gel bands would the probe above detect on a Southern blot of an individual heterozygous for the polymorphic restriction site? Put a number in the box.

Answer 1

1) That restriction site is a SNP.

That restriction site is a RFLP.

Explanation: Sometimes, SNP in a genome can make a site as a restriction site, which was not, before the SNP.

2) Three gel bands would the probe above detect on a Southern blot of an individual heterozygous for the polymorphic restriction site.

Explanation: As the individual is heterozygous, he would have one gel band that does not contain the same pattern of restriction site. So, the probe can hybridize with that. The second DNA that has the above mentioned restriction site pattern, would generate more than four fragments after restriction digestion. Among them two fragments will hybridize with probe.