Question

PROCEDURE 1. Prepare three 100 mL beakers for this experiment. Use the molecular weights on the bottles to calculate the amount of solid to use. a. *b. Prepare 25 mL of a 10 M NaOH solution (concentrated NaOH solution). Calculate the amount of solid NaOH needed to make. *c. Prepare 50 mL of a 20 mM histidine solution (calculate the amount of solid histidine needed to make it, m.w. 209.6 g/mol). In the fume hood, add 3 drops of concentrated HCI to the amino acid solution to further acidify it. This is done so that we will be able to see more of the titration curve. 50 mL of distilled water. In the fume hood, add 3 drops of concentrated HCI to the water to acidify it. d. Note: NaOH solution is much more concentrated than the amino acid solution. This will allow you to titrate the amino acid by adding very small amounts of NaOH. Calibrate the pH probe and then rinse the probe with fresh water. 2.

Answer 1

Molecular weight of NaOH= (23+16+1 Molai mass - 40 q.not 25 ml 10 M Naott 2.5 x 10 ml 250 ml.m»l NaoH 250. ml. mol 10 m 25 mol NaoH .' 40 q s the mass solid NaOH is meeded

Molecu loJi ueig/hto his idine 2096 -1 2o m histidime solwior 50 ml 3 3 ml. hishdime 50× 20x10 mot histhdime - 1C shown It has bee o mo histidime 26 q s the mass o 1 mol sf histidine 2016 q hstidime So' o. 20% g histīdine is required