Question

84) Refer to the following gels and answer the questions below (7 pts) B. 1) "non-GMO" test sample, plant mastermix 2) "non-GMO test sample, GMO mastermix 3) "GMO' test sample, plant mastermix 4) "GMO test sample, GMO mastermix 5) GMO purified DNA, plant mastermix. 6) GMO purified DNA, GMO mastermix 7) Molecular weight marker Which gel has the pattern you would predict to see if: A) The DNA extraction didn't work? B) The test food was a GMO and the non-GMO was not? C) The PCR machine didn't work? D) The test food was not a GMO but the non-GMO food was? E) The test food and the non-GMO samples both were actually GMO? F) Neither the test food nor the non-GMO samples were GMO? G) The PCR mastermixes were contaminated with DNAse?

Answer 1

Q.A

In gel A there is not band on gel other than DNA marker. It means DNA extraction didnt work and there is no DNA in sample.

Q.B

Gel D shows that test food was GMO but non GMO was not, thats why it did not gave amplification during PCR and well no. 4 is not visible here, while well no 2 is visible here.

Q.C

In Gel B PCR machine didnt work properly, because of which DNA in test sample was not amplified and visible in gel. Since no. 5 and 6 well have purified DNA, it will be clearly visible.

Q.D

Gel C shows that test food was not GMO but non GMO was , thats why it did not gave amplification during PCR and well no. 2 is not visible here, while well no 4 is visible here.

Q.E

In gel F both samples were GMO, Thats why they gave amplification in PCR analysis.

Q.F
Gel E shows that none of the samples were GMO, that's why they did not gave amplification during analysis. Well no.1,3, 5 and 6 gave amplification because they contained GMO DNA.

Q.G

In Gel A , none of the mastermix have DNA which means DNA in these samples were likely destroyed by DNAse.