Question

o) A two stage chemostate system is used for production of se 3 ry metabolite. The volume of each reactor is 2 m'; the flow rate of feed is 200 ocurs in the both ractors but the scecond reactor is used for L/h. Mycelial growth for product synthesis. The concentration of substrate in the feed is 20 g/1. Kinetic and yield parameters for the organisms are: Yxs = 0.5 kg/kg Ks 1.0 kg/m Hm 0.12/h ms = 0.025 kg/kg-h p 0.16kg/kg-h Yps 0.85kg/kg Assume that product synthesis is negligible in the first reactor. i. Determine the cell concentration entering the second reactor. ii. What is the final concentration of the product?

Answer 1

Amsuer Volume of each reactor: 2m2 = V How rate feel200L 3 z 20 10 - 6.85 PS FL贉勁 Fi.xo, So V, H 2X Assume, H=H(x) Cel mass beaee 0) 巩

for subtrate balane alt for ' XS P S F S os F xS ps 200 X1δ3 ( 20 9-99X10 2 6.8S O.S Coventrakiom) - ra comcon fors ubstrate (S)ー 一.3 90.gx10 %,3 for Cell (*)-8.377 65C kac