Question

218 Unit IV: Special Procedures SKILLS DRILL 11-3: BLOOD CULTURE SPECIMEN COLLECTION Instructions: Match the rationale with the corresponding step in the procedure. Procedure Step Rationale A. Antisepsis does not occur instantly B. Notation of site location is necessary because there may be an isolated infection in that area. C. Ensuring antiseptic technique and sterility of the site te critical to accurate diagnosis. D. The CLSI standard states that the tourniquet should not be left on longer than 1 minute E. Inoculation of the medium can occur directly into the bottle or after collection when a syringe is used. F. Blood culture bottles have vacuum, but it is not always 1. . Identify venipuncture site and release tourniquet 2. Aseptically select and assemble equipment. 3. Perform friction scrub as prescribed. 4. Allow site to air-dry. Cleanse the culture bottle stoppers while the site is drying. measured as in evacuated tubes. G. Aseptic technique reduces the risk of false positives due to contamination H. The tops of the culture bottles must be free of contami nants when they are inoculated. I. Bacteria exist on the skin surface and can be removed temporarily 6. Mark the minimum and maximum fill on the culture bottles. Reapply tourniquet and per- form venipuncture without touching the site. 7. Inoculate the media bottles as required. 8 Label the specimen containers with required ID, including the site of collection.

Answer 1

1o D - The CL 31 standard states that the tourniquet should not be left no - longer than I minite. 2 G - Aseptic technique reduces the risk - of false positives due to contamination. 3. I - Bacteqia exist on the skin surface and can be removed temporarily. 4. A - Antiseptics does not occur instantly. 5. H - Tops of culture bottles must be free of contaminants cohen they are inoculated. 6.5 - Blood culture bottles have vaccum, but it is not always measured as in evaluated tubes- 7.5. Ensuring antiseptic technique and sterility of the site is critical to accurate diagnosis 8 E. Inoculation of the medium can occur directly into the bottle or after collection when a syringe is used: 9. B Notation of site location is necessary because there may be an isolated infection in that area.