Generally, what is the ideal Rf value of the analyte when using TLC to choose a solvent for column chromatography?
Generally, what is the ideal Rf value of the analyte when using TLC to choose a solvent for column chromatography?
Homework Answers
Rf value is the ratio between distance traveled by a given component and distance traveled by the solvent front.
An ideal lies between 0.3 to 0.7 since it is likely that other compounds present in the mixture will be visible on the TLC plate when the Rf is in this range.
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Generally, what is the ideal Rf value of the analyte when using TLC to choose a solvent for column chromatography?
Problem 1 Thin layer Chromatography consist of three parts: The analyte, the stationary phase and mobile...
Problem 1 Thin layer Chromatography consist of three parts: The analyte, the stationary phase and mobile phase. Match each of these terms to what is was in out experiment. Stationary Phase ____ a) The solvent Mobile Phase ____ b) Silica Analyte ____ c) One of the analgesiscs Problem 2 Complete the sentences Analytes on a TLC should have a(n) _________ Rf in a less polar solvent. Analytes on a TLC should have a(n) _________ Rf in a more polar solvent....
What is the main purpose of running a column chromatography? Similar to TLC. column chromatography is...
What is the main purpose of running a column chromatography? Similar to TLC. column chromatography is based on analytes being partitioned between a stationary phase (not moving) and a mobile phase (moving). What is most commonly used as stationary phase in a column? Is this material polar or non-polar? In this experiment, you will use different solvents to run column chromatography separation of different pigments extracted from spinach leaves. These solvents are a mixture of acetone and hexanes. (a) What...
1. What are the main differences between thin layer chromatography (TLC) and column chromatography (CC)? 2. Are CC...
1. What are the main differences between thin layer chromatography (TLC) and column chromatography (CC)? 2. Are CC and TLC operations conducted concurrently? Explain. 3. Name a few packing materials used in packing columns in Column Chromatography operations. 4. Explain the role of eluting solvent(s) in column chromatography procedures. 5. When performing column chromatography, which of the following compounds would you expect to elute first: Ferrocene of acetylferrocene? Explain why.
2. (5) How is column chromatography related to TLC? Gas chromatography? Be sure to discuss mobile...
2. (5) How is column chromatography related to TLC? Gas chromatography? Be sure to discuss mobile phase, stationary phase, sample size, sample type in your answer. 3. (2) How is Rf in TLC related to elution order in column chromatography? What do you expect to come out first and last?
Thin layer chromatography 6. When should a mixed-solvent system be used for TLC? Explain. 7. Is...
Thin layer chromatography
6. When should a mixed-solvent system be used for TLC? Explain. 7. Is the order of elution of the components the same for TLC as for column chro- matography? Explain bow.the various fractions can be analyzed from a gravity column.
For the column chromatography experiment - After separation, analyze your results by TLC. Draw the TLC...
For the column chromatography experiment - After separation, analyze your results by TLC. Draw the TLC plate results below (actual size). Calculate Rf for each spot. Label identity of each spot.
Describe the differences between paper chromatography and TLC. Give two advantages and two disadvantages of each...
Describe the differences between paper chromatography and TLC. Give two advantages and two disadvantages of each method for analysis. In order to prepare for a column chromatography separation of 3 ink dye components, you have run a TLC experiment with three possible solvent mixtures. These are the results: Acetone:Hexane in 1:1 ratio gave the following distances: solvent=4.2cm, pigment #1=3.8cm, pigment #2=1.7cm, and pigment #3=1.3cm Acetone:Hexane in 2:1 ratio gave the following distances: solvent=4.3cm, pigment #1=3.8cm, pigment #2=2.1cm, and pigment...
In thin layer chromatography, would a more polar solvent increase or decrease the Rf values. Explain...
In thin layer chromatography, would a more polar solvent increase or decrease the Rf values. Explain your answer by referring to TLC theory (stationary phase, moving phase, polarity...)
indicate the differences in when each of column chromatography and TLC is being used.
indicate the differences in when each of column chromatography and TLC is being used.
A chemist runs a thin–layer chromatography (TLC) plate and, after development, forgets to mark the solvent...
A chemist runs a thin–layer chromatography (TLC) plate and, after development, forgets to mark the solvent front. The chemist doesn\'t realize this until the solvent has evaporated. Assuming that accurate Rf values are needed, select the correct course of action for the chemist. a. Assume that the solvent front is at the top of the plate. b. Dip the plate in phosphomolybdic acid (PMA) and heat the plate with a heat gun. c. Run the TLC analysis of these samples...
What is the main purpose of running a column chromatography? Similar to TLC. column chromatography is based on analytes being partitioned between a stationary phase (not moving) and a mobile phase (moving). What is most commonly used as stationary phase in a column? Is this material polar or non-polar? In this experiment, you will use different solvents to run column chromatography separation of different pigments extracted from spinach leaves. These solvents are a mixture of acetone and hexanes. (a) What...
1. What are the main differences between thin layer chromatography (TLC) and column chromatography (CC)? 2. Are CC and TLC operations conducted concurrently? Explain. 3. Name a few packing materials used in packing columns in Column Chromatography operations. 4. Explain the role of eluting solvent(s) in column chromatography procedures. 5. When performing column chromatography, which of the following compounds would you expect to elute first: Ferrocene of acetylferrocene? Explain why.
2. (5) How is column chromatography related to TLC? Gas chromatography? Be sure to discuss mobile phase, stationary phase, sample size, sample type in your answer. 3. (2) How is Rf in TLC related to elution order in column chromatography? What do you expect to come out first and last?
Thin layer chromatography
6. When should a mixed-solvent system be used for TLC? Explain. 7. Is the order of elution of the components the same for TLC as for column chro- matography? Explain bow.the various fractions can be analyzed from a gravity column.
For the column chromatography experiment - After separation, analyze your results by TLC. Draw the TLC plate results below (actual size). Calculate Rf for each spot. Label identity of each spot.
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