Transcriptomics uses what following technology:
RNAseq
Whole genome sequencing
Mass Spectrometry
Microarray
ANSWER
* RNA-seq or RNA sequencing uses next generation sequencing to expose the quantity of RNA
in a biological sample at a particular moment and helpful to analyse the continous changing
transcriptome.
Transcriptomics uses what following technology: RNAseq Whole genome sequencing Mass Spectrometry Microarray
Transcriptomics uses following technology: RNAseq Whole genome sequencing Mass Spectrometry Microarray A-All of the above b.1,2 and 4 c. 3 and 4 d. 1 and 4
Diagram a basic outline of the process of whole-genome sequencing to demonstrate that you understand key steps involved in sequencing a large genome.
To sequence the whole genome of all the organisms present in a sample, you would use PCR, followed by cloning, followed by Sanger sequencing PCR, followed by DGGE, followed by Sanger sequencing CARD-FISH Nanopore technology
Select the steps that occur during whole-genome shotgun sequencing. Check All That Apply Whole genomes are broken down into small DNA fragments. DNA fragments are separated by the process of membrane filtration DNA fragments are cloned into bacterial cells to create a library. Contigs are ordered to create a complete genomic sequence.
How has DNA-sequencing technology evolved in response to the emerging needs of genome scientists? Drag the terms on the left to the appropriate blanks on the right to complete the sentences. Not all terms will be used. Reset Help reduce speed sequencing methods, a single serves as a template. In one such strategy, the DNA polymerase is tethered in a nanopore and fluorescently labeled nucleotides are detected as they are incorporated. The overall goal is to and accuracy of sequencing...
What is cloning? Why is it necessary to clone genomic DNA fragments when sequencing a genome by the Sanger Shotgun approach?
Mass Spectrometry What is the importance of using the technique & what questions does Mass Spectrometry help us answer?
Multiple choice (Choose as many as you believe to be correct) 1. If you have 45 melanoma surviving patients and 20 melanoma deceased patients, your goal is to identify what genes are associated with cancer development and resistance. Which experiments cannot fulfil your goal? A. Microarray. B. RNA-Seq. C. Mass Spectrometry. D. RT-PCR. 2. Which of the sequencing technology is likely to have the longest read length? A. Illumina HiSeq2500. B. PacBioRS. C. ABI SOLiD. D. IonTorrent.
1st attempt When using tandem mass spectrometry for peptide sequence determination, what is the input into the second mass spectrometer from the collision chamber? Choose one: A. Peptides from a select range of masses B. Tryptic fragments of the protein of interest C. Isolated and fragmented peptides D. Whole protein
Based on mass spectrometry, infrared spectroscopy, H NMR and
CNMR, what could the following unknown be?
Mass Spectrometry (not shown): [M] = 156 (75%), [M+2] = 158 (100%), [M+4] = 160 (25%) m/z Infrared Spectroscopy (not shown): 2966, 2910 cm H Nuclear Magnetic Resonance. quin 2 2H PPM 1 0 4 2H 2H 13C Nuclear Magnetic Resonance. 20 35 25 PPM 15 45 40 30 10