Homework Answers
Cloning, in terms of genetic engineering, is insertion of a small DNA fragment into circular DNA molecules called plasmids. This is especially important during sequencing of DNA using Sanger shotgun sequencing, because this sequencing method can only sequence DNA fragments of 100-1000bps at a stretch. Hence the genome needs to be broken into fragments which are cloned in separate vector and then sequenced.
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What is cloning? Why is it necessary to clone genomic DNA fragments when sequencing a genome...
Select the steps that occur during whole-genome shotgun sequencing. Check All That Apply Whole genomes are...
Select the steps that occur during whole-genome shotgun sequencing. Check All That Apply Whole genomes are broken down into small DNA fragments. DNA fragments are separated by the process of membrane filtration DNA fragments are cloned into bacterial cells to create a library. Contigs are ordered to create a complete genomic sequence.
Place the steps involved in the "shotgun" approach to sequencing a genome in the correct order
Place the steps involved in the "shotgun" approach to sequencing a genome in the correct order Assign scaffolds to known chromosomal sites. Break the whole genome into fragments. Reconstruct scaffolds from overlapping sequences.Sequence fragments with the shotgun approach.
1. Assemble a 24 base DNA sequence from these sequence fragments, produced by shotgun sequencing? (Don't...
1. Assemble a 24 base DNA sequence from these sequence fragments, produced by shotgun sequencing? (Don't forget that sequences can occur from either direction with shotgun sequencing). GATGAC GACATGGC TCAGTCGA CGATGCG GGCGTCAG
Genomic libraries are constructed for genomic sequencing. The library of vectors is transformed into bacteria or...
Genomic libraries are constructed for genomic sequencing. The library of vectors is transformed into bacteria or yeast. Each bacterial or yeast cell grows into a colony and represents one unique fragment of DNA. Suppose you are working with a newly discovered bacteria with a genome of 5290 kbp. If you construct a genomic library made up of 15 kbp fragments, how many clones would you have to screen to get a 99% probability of finding a particular fragment? number of...
Genomic libraries are constructed for genomic sequencing. The library of vectors is transformed into bacteria or...
Genomic libraries are constructed for genomic sequencing. The library of vectors is transformed into bacteria or yeast. Each bacterial or yeast cell grows into a colony and represents one unique fragment of DNA. Suppose you are working with a newly discovered bacteria with a genome of 4850 kbp. If you construct a genomic library made up of 8.0 kbp fragments, how many clones would you have to screen to get a 99% probability of finding a particular fragment? number of...
When would you want to use Shotgun Sequencing? a. Whenever both strands of the DNA are...
When would you want to use Shotgun Sequencing? a. Whenever both strands of the DNA are being sequenced in the same reaction tube(s). b. When radioactive nucleotides are to be used to terminate replication. c. When a large piece of DNA (like a genome) needs to be sequenced. d. Whenever the DNA sequence is unknown.
The Sanger method of DNA sequencing uses which chain-terminating ingredient to separate fragments differing by a...
The Sanger method of DNA sequencing uses which chain-terminating ingredient to separate fragments differing by a single nucleotide a. Magnesium b. Heat c. ddNTPs d. restriction enzymes e. none of the above
Sanger sequencing or dideoxy sequencing of DNA causes termination of replication. Why? 18.
Sanger sequencing or dideoxy sequencing of DNA causes termination of replication. Why? 18.
please help with all three! For DNA synthesis to take place in a Sanger sequencing reaction,...
please help with all three!
For DNA synthesis to take place in a Sanger sequencing reaction, the DNA polymerase must catalyze a reaction between the 3'- the last nucleotide and the 5- of the next nucleotide to be added. hydroxyl group, phosphate group O phosphate group, hydroxyl group O hydroxyl group, hydroxyl group O phosphate group, phosphate group QUESTION 6 millions of individual DNA fragments are isolated and sequenced in parallel during each machine run. O traditional whole-genome sequencing O...
command 145 Genetics Assignment Genomic Analysis 1. Some bacteria normally produce endonucleases for what purpose? a)...
command 145 Genetics Assignment Genomic Analysis 1. Some bacteria normally produce endonucleases for what purpose? a) necessary digestion of their own genome b) defense against viral infection c) bacteria never make endonucleases naturally 2. A blunt cutter produces DNA fragments with complementary overhanging regions. a) True b) False 3. Which of the following DNA sequences (when double-stranded) most likely represents a restriction endonuclease site for a 6-mer cutter? a) AGTAAGCTTC c) AGAGAGCCAA b) GGTAGATTCC d) None of the above 4....
Select the steps that occur during whole-genome shotgun sequencing. Check All That Apply Whole genomes are broken down into small DNA fragments. DNA fragments are separated by the process of membrane filtration DNA fragments are cloned into bacterial cells to create a library. Contigs are ordered to create a complete genomic sequence.
Genomic libraries are constructed for genomic sequencing. The library of vectors is transformed into bacteria or yeast. Each bacterial or yeast cell grows into a colony and represents one unique fragment of DNA. Suppose you are working with a newly discovered bacteria with a genome of 5290 kbp. If you construct a genomic library made up of 15 kbp fragments, how many clones would you have to screen to get a 99% probability of finding a particular fragment? number of...
Genomic libraries are constructed for genomic sequencing. The library of vectors is transformed into bacteria or yeast. Each bacterial or yeast cell grows into a colony and represents one unique fragment of DNA. Suppose you are working with a newly discovered bacteria with a genome of 4850 kbp. If you construct a genomic library made up of 8.0 kbp fragments, how many clones would you have to screen to get a 99% probability of finding a particular fragment? number of...
Sanger sequencing or dideoxy sequencing of DNA causes termination of replication. Why? 18.
please help with all three!
For DNA synthesis to take place in a Sanger sequencing reaction, the DNA polymerase must catalyze a reaction between the 3'- the last nucleotide and the 5- of the next nucleotide to be added. hydroxyl group, phosphate group O phosphate group, hydroxyl group O hydroxyl group, hydroxyl group O phosphate group, phosphate group QUESTION 6 millions of individual DNA fragments are isolated and sequenced in parallel during each machine run. O traditional whole-genome sequencing O...
command 145 Genetics Assignment Genomic Analysis 1. Some bacteria normally produce endonucleases for what purpose? a) necessary digestion of their own genome b) defense against viral infection c) bacteria never make endonucleases naturally 2. A blunt cutter produces DNA fragments with complementary overhanging regions. a) True b) False 3. Which of the following DNA sequences (when double-stranded) most likely represents a restriction endonuclease site for a 6-mer cutter? a) AGTAAGCTTC c) AGAGAGCCAA b) GGTAGATTCC d) None of the above 4....
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