Place the steps involved in the "shotgun" approach to sequencing a genome in the correct order
Place the steps involved in the "shotgun" approach to sequencing a genome in the correct order
Assign scaffolds to known chromosomal sites.
Break the whole genome into fragments.
Reconstruct scaffolds from overlapping sequences.
Sequence fragments with the shotgun approach.
Homework Answers
1. Break the whole genome into fragments.
2. Reconstruct scaffolds from overlapping sequences
3. Sequence fragments with the shotgun approach
4. Assign scaffolds to known chromosomal sites.
The answers on the top is wrong.
break
sequence
reconstruct
assign
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Place the steps involved in the "shotgun" approach to sequencing a genome in the correct order
Select the steps that occur during whole-genome shotgun sequencing. Check All That Apply Whole genomes are...
Select the steps that occur during whole-genome shotgun sequencing. Check All That Apply Whole genomes are broken down into small DNA fragments. DNA fragments are separated by the process of membrane filtration DNA fragments are cloned into bacterial cells to create a library. Contigs are ordered to create a complete genomic sequence.
please help with all three! For DNA synthesis to take place in a Sanger sequencing reaction,...
please help with all three!
For DNA synthesis to take place in a Sanger sequencing reaction, the DNA polymerase must catalyze a reaction between the 3'- the last nucleotide and the 5- of the next nucleotide to be added. hydroxyl group, phosphate group O phosphate group, hydroxyl group O hydroxyl group, hydroxyl group O phosphate group, phosphate group QUESTION 6 millions of individual DNA fragments are isolated and sequenced in parallel during each machine run. O traditional whole-genome sequencing O...
Place the following images into the correct order to represent the sequence of the steps of...
Place the following images into the correct order to represent the sequence of the steps of endochondral ossification.
Place the following steps of DNA replication in order from the beginning to the end of...
Place the following steps of DNA replication in order from the beginning to the end of the process. Your answer will be a series of numbers (1-5) in what you think is the correct order. 1. Joining of Okazaki fragments by DNA ligase 2. Priming of DNA synthesis with RNA primer 3. Opening and stabilizing the DNA strands 4.removal of primers by DNA polymerase lexonuclease 5. elongation of RNA primers by DNA polymerase III
Place the following steps of TRANSLATION in the correct order for EUKARYOTES. The ribosome reaches a...
Place the following steps of TRANSLATION in the correct order for EUKARYOTES. The ribosome reaches a stop codon. A release factor binds and causes the release of the new polypeptide, along with the mRNA. The ribosome dissociates. v Acharged tRNA with a matching anticodon binds the mRNA codon in the A site. ✓ The ribosome moves exactly 3 nucleotides toward the 3* end of the mRNA. The small ribosomal subunit uses rRNA to bind to the Kozak sequence, which places...
Below is a series of events involved in the mechanism of forming a retrotransposon. Place these...
Below is a series of events involved in the mechanism of forming a retrotransposon. Place these steps in the correct order 1. the DNA copy is made double-stranded 2. DNA of the transposable element is transcribed 3. The DNA of the transposable element is integrated into a target DNA site 4. The RNA is reverse transcribed by reverse transcriptase, producing a complementary DNA 4,2,3,1 3,2,4,1 2,4,1,3 4,2,1,3 1,2,3,4 What is the function of the poly(A) tail on most mRNAs To...
A cell's genome is its blueprint for life. However, what is the bare minimum number of...
A cell's genome is its blueprint for life. However, what is the bare minimum number of genes needed to sustain a free-living cell? This is a question that microbiologists at the J. Craig Venter Institute (JCVI) have attempted to answer ever since they sequenced the genomes of several Mycoplasma species in the 1990s. Because Mycoplasma species are parasitic bacteria, their genomes are already reduced in size and hence provide an excellent foundation for creating a "minimal cell." However, little did...
and w Two-dimensional gel electrophoresis separates proteins based on a. shape; charge Ob.size; concentration c. concentration;...
and w Two-dimensional gel electrophoresis separates proteins based on a. shape; charge Ob.size; concentration c. concentration; shape O d. size, charge O e. size; shape Refer to the table. Several strains of a bacterium are sequenced to investigate the pan and core genomes. In the table, + denotes presence of the gene and denotes its absence. Gene Gene Gene Gene Gene Strain ! Strain 2 + Strain 3 + Strain 4 + + + + Strain 5 + + What...
Quiz Cell Respirationi Saved Help Save & Exit Submit Place the steps in the correct order...
Quiz Cell Respirationi Saved Help Save & Exit Submit Place the steps in the correct order to describe the stages of the citric acid cycle per turn. Twice over, substrates are oxidized as NAD+ is reduced to NADH and CO, is released. NADH Again a substrate is oxidized, but this ime FAD is reduced to FADH C, 2 The cycle begins when an acetyl group carried by CoA combines with a C4 molecule to form citrate acetyl CoA Citric acid...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving rise to a “hairless” phenotype. In the homozygous condition, H is lethal. An independently assorting dominant allele S has no effect on bristle number except in the presence of H, in which case a single dose of S suppresses the hairless phenotype, thus restoring the "hairy" phenotype. However, S also is lethal in the homozygous (S/S) condition. What ratio of hairy to hairless flies...
Select the steps that occur during whole-genome shotgun sequencing. Check All That Apply Whole genomes are broken down into small DNA fragments. DNA fragments are separated by the process of membrane filtration DNA fragments are cloned into bacterial cells to create a library. Contigs are ordered to create a complete genomic sequence.
please help with all three!
For DNA synthesis to take place in a Sanger sequencing reaction, the DNA polymerase must catalyze a reaction between the 3'- the last nucleotide and the 5- of the next nucleotide to be added. hydroxyl group, phosphate group O phosphate group, hydroxyl group O hydroxyl group, hydroxyl group O phosphate group, phosphate group QUESTION 6 millions of individual DNA fragments are isolated and sequenced in parallel during each machine run. O traditional whole-genome sequencing O...
Place the following images into the correct order to represent the sequence of the steps of endochondral ossification.
Place the following steps of DNA replication in order from the beginning to the end of the process. Your answer will be a series of numbers (1-5) in what you think is the correct order. 1. Joining of Okazaki fragments by DNA ligase 2. Priming of DNA synthesis with RNA primer 3. Opening and stabilizing the DNA strands 4.removal of primers by DNA polymerase lexonuclease 5. elongation of RNA primers by DNA polymerase III
Place the following steps of TRANSLATION in the correct order for EUKARYOTES. The ribosome reaches a stop codon. A release factor binds and causes the release of the new polypeptide, along with the mRNA. The ribosome dissociates. v Acharged tRNA with a matching anticodon binds the mRNA codon in the A site. ✓ The ribosome moves exactly 3 nucleotides toward the 3* end of the mRNA. The small ribosomal subunit uses rRNA to bind to the Kozak sequence, which places...
Below is a series of events involved in the mechanism of forming a retrotransposon. Place these steps in the correct order 1. the DNA copy is made double-stranded 2. DNA of the transposable element is transcribed 3. The DNA of the transposable element is integrated into a target DNA site 4. The RNA is reverse transcribed by reverse transcriptase, producing a complementary DNA 4,2,3,1 3,2,4,1 2,4,1,3 4,2,1,3 1,2,3,4 What is the function of the poly(A) tail on most mRNAs To...
and w Two-dimensional gel electrophoresis separates proteins based on a. shape; charge Ob.size; concentration c. concentration; shape O d. size, charge O e. size; shape Refer to the table. Several strains of a bacterium are sequenced to investigate the pan and core genomes. In the table, + denotes presence of the gene and denotes its absence. Gene Gene Gene Gene Gene Strain ! Strain 2 + Strain 3 + Strain 4 + + + + Strain 5 + + What...
Quiz Cell Respirationi Saved Help Save & Exit Submit Place the steps in the correct order to describe the stages of the citric acid cycle per turn. Twice over, substrates are oxidized as NAD+ is reduced to NADH and CO, is released. NADH Again a substrate is oxidized, but this ime FAD is reduced to FADH C, 2 The cycle begins when an acetyl group carried by CoA combines with a C4 molecule to form citrate acetyl CoA Citric acid...
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