Genomic libraries are constructed for genomic sequencing. The library of vectors is transformed into bacteria or...
Genomic libraries are constructed for genomic sequencing. The library of vectors is transformed into bacteria or yeast. Each bacterial or yeast cell grows into a colony and represents one unique fragment of DNA. Suppose you are working with a newly discovered bacteria with a genome of 5290 kbp. If you construct a genomic library made up of 15 kbp fragments, how many clones would you have to screen to get a 99% probability of finding a particular fragment? number of...
NOT 2033 someone already came up with that answer and its wrong there is a question... theres an entire paragraph Genomic libraries are constructed for genomic sequencing. The library of vectors is transformed into bacteria or yeast. Each bacterial or yeast cell grows into a colony and represents one unique fragment of DNA. Suppose you are working with a newly discovered bacteria with a genome of 4850 kbp. If you construct a genomic library made up of 8.0 kbp fragments,...
You need to prepare sequencing libraries of two different DNA fragment sizes. One library should contain DNA fragments of approximately 800 bp and the other 5000 bp. You are going to fragment your DNA using a nebulizer. Indicate the correct statement(s) below: a. To obtain a library of 800 bp fragments, you need to apply lower gas pressure during the nebulization process than for the preparation of the 5000 bp fragment library. b. To obtain a library of 5000 bp...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving rise to a “hairless” phenotype. In the homozygous condition, H is lethal. An independently assorting dominant allele S has no effect on bristle number except in the presence of H, in which case a single dose of S suppresses the hairless phenotype, thus restoring the "hairy" phenotype. However, S also is lethal in the homozygous (S/S) condition. What ratio of hairy to hairless flies...