Question

You need to prepare sequencing libraries of two different DNA fragment sizes. One library should contain DNA fragments of approximately 800 bp and the other 5000 bp. You are going to fragment your DNA using a nebulizer. Indicate the correct statement(s) below:

a.   To obtain a library of 800 bp fragments, you need to apply lower gas pressure during the nebulization process than for the preparation of the 5000 bp fragment library.

b.   To obtain a library of 5000 bp fragments, you need to apply lower gas pressure during the nebulization process than for the preparation of the 800 bp fragment library.

c.   The pressure of the gas used during nebulization does not influence the size of the DNA fragments.

d.   The size of the DNA fragments obtained in the process of nebulization depends on the GC content of the DNA.

e.   The size of sheared DNA molecules is inversely proportional to the size of the droplets formed during the process of nebulization.

f.    I do not know anything about the nebulization of DNA, so I cannot evaluate any of the above statements.