Question

3. Below is a Lineweaver-Burke plot of an enzyme reaction in the presence and absence of an inhibitor. 2.4 2.3 2.2 2.1 2 1.9 1.8 1.7 1.6 1.5 1.4 1.3 1.2 1.1 1 0.9 0.8 < 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0 -0.1 -0.2 -0.3 -0.4 -0.5 -0.6 -0.7 -0.8 . . . . . -1 -0.9 -0.8 -0.7 -0.6 -0.5 -0.4 -0.3 -0.2 -0.1 O 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 1.1 1/[S] (1/nM) a. What is Vmax,app for both reactions? b. What is K Mapp for both reactions? c. What sort of inhibition is occurring? d. A doctor prescribed the inhibitor to a person and then told that person to also avoid eating foods that contained a lot of the substrate for the enzyme. Explain why (in terms of the enzyme, inhibitor, and substrate)

Answer 1

3. Below is a Lineweaver-Burke plot of an enzyme reaction in the presence and absence of an inhibitor. x intercept = -1/km y intercept = 1/Vmax 1/v (s) 2.4 2.3 2.2 2.1 2 1.9 1.8 1.7 1.6 1.5 1.4 1.3 1.2 1.1 1 U 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 0 0.1 -0.2 -0.3 -0.4 -0.5 -0.6 -0.7 -0.8 -1 -0.9 -0.8 -0.7 -0.6 -0.5 -0.4 -0.3 -0.2 -0.1 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 1.1 1/[(1/nM) a. What is Vmax,app for both reactions? 1/Vmax=0.2 Vmax = 1/0.2 = 5 (for both) red line, -1/km = -0.1 b. What is K Mapp for both reactions? km = 1/0.1 = 10 blue line, -1/km = -0.4 km = 1/0,4 = 2.5 c. What sort of inhibition is occurring? Vmax unchanged, Km increasing competitive

D. The inhibitor will act as a medicine for the person and will help in the prevention of enzyme binding to the substrate and will give a relaxation to the patient. However, if the patient will continue taking food which are not recommended by the doctor, then he is actually increasing the substrate concentration which will be easily acted upon by the enzyme. Enzymes cannot bind to the substrate in the presence of inhibitor but the effect can be reversed if the concentration of substrate is increased.

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