When would you want to use Shotgun Sequencing? a. Whenever both strands of the DNA are...
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When would you want to use Shotgun Sequencing?
a. Whenever both strands of the DNA are being sequenced in the same reaction tube(s).
b. When radioactive nucleotides are to be used to terminate replication.
c. When a large piece of DNA (like a genome) needs to be sequenced.
d. Whenever the DNA sequence is unknown.
Homework Answers
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When would you want to use Shotgun Sequencing?
a.
Whenever both strands of the DNA are...
e. of ninot0 AOLL 2. A microarray is an orderly arrangement of short strands of DNA...
e. of ninot0 AOLL 2. A microarray is an orderly arrangement of short strands of DNA attached to a solid surface. Considering SNP microarrays, expression arrays and CNV arrays (array comparative genome hybridization): a. Which type of array(s) will not have intronic sequences attached to the solid surface? Why? b. Which type of array will have a larger proportion of the genome attached to the solid surface? Why? Which type of array would allow one to identify large stretches of...
Assume you are working to sequence the genome of a newly identified alien species. The alien...
Assume you are working to sequence the genome of a newly identified alien species. The alien DNA is composed of the same nucleotides are in us and, its genome is 2-3 times larger than that of any terrestrial organism, and this genome is contained within 2 huge linear chromosomes and once smaller circular chromosome. Assume , mechanisms of biology (i.e. transcription, translation, replication, etc. are similar to those acting in terrestrial eukaryotes. That said, here is no reason to believe...
The following diagram represents a replication bubble associated with DNA synthesis. Based on this diagram, select...
The following diagram represents a replication bubble associated
with DNA synthesis. Based on this diagram, select all of the
options below that are true.
1 | 2 ---- Quadrants 1 and 4 are associated with lagging strand synthesis Quadrants 1 and 4 are associated with leading strand synthesis Quadrants 1 and 2 are associated with lagging strand synthesis Quadrants 3 and 4 are associated with lagging strand synthesis Synthesis of both daughter strands is completely continuous Telomerase activity is needed...
If you wish to sequence a long strand of DNA in one round of reactions, you...
If you wish to sequence a long strand of DNA in one round of reactions, you should: O A. Decrease the ddNTP/dNTP ratio O B. Increase the ddNTP/dNTP ratio O C. Use a shorter DNA primer O D. Add twice as much primer Based on this figure, the most likely error is: O A. The scientist forgot to add dNTPs to one of the reaction tubes. O O B. <label for="q7_4" id="lq7_4">The scientist did not denature the DNA strands</label> O...
Next-generation sequencing involves: A. generating many short sequences from an intact, continuous DNA sequence. B. generating...
Next-generation sequencing involves: A. generating many short sequences from an intact, continuous DNA sequence. B. generating many short sequences from fragmented DNA. C. splicing together DNA fragments. D. adding multiple probes to fragmented DNA. What is the difference between a mutation and a polymorphism? A. A mutation can exist in a single person; a polymorphism must exist in at least 2% of the population. B. A mutation involves a single base pair; a polymorphism involves many base pairs. C. Mutations...
DNA DNA Replication: ONA Because DNA Is the ge m Tumes and heart e ine in...
DNA DNA Replication: ONA Because DNA Is the ge m Tumes and heart e ine in process called DNA curs in the nucleus of s acest FS Parent strand Parent strand Newly replicated DNA Newly replicated DNA- SA0 Daughter DNA molecule Daughter DNA molecule Figure 8.2: Overview of DNA replication and illustration of complementary base pairing. DNA must replicate before cell division so that each new daughter cell receives an exact copy of the parent DNA. 1. Replication begins when...
1. the genes that seem to be the most necessary to maintain, since they are present...
1. the genes that seem to be the most necessary to maintain, since they are present in the smallest cellular organisms are a. cytoskeletal protein genes b, translation protein genes c. replication protein genes d. transcription protein genes e. DNA repair protein genes 2. Why do cellular organisms generally look very similar when early embryos but different from each other when mature? a. their DNAs have different chemistry b. their RNAs are different lengths c. what genes get turned on...
Question 7 estion 7 0.3125 points Suppose that in a Sanger sequencing reaction, sequences are readable...
Question 7 estion 7 0.3125 points Suppose that in a Sanger sequencing reaction, sequences are readable for a few dozen nucleotides but then become increasingly difficult to road. It is determined that the chains are terminated to readily. What is the most likely cause? a. Too much of the dNTPs b. Insufficient fluorescent dye Too much fluorescent dye d. Incorrect primer e. Insufficient dNTPs Question of 20 Moving to another question will save this response Question 13 Which statement comparing...
You isolate plasmid DNA from bacteria (Questions 7-10) 7) A plasmid is an extrachromosomal circul...
10 please and 7
You isolate plasmid DNA from bacteria (Questions 7-10) 7) A plasmid is an extrachromosomal circular DNA frequently found in prokaryotes. Aside from being smaller, how is it different from the prokaryotic genome? You place equal amounts of plasmid DNA in 4 different tubes and incubate the DNA with increasing amounts of the enzyme topoisomerase I for 1 hour (0 enzyme units 0.25 enzyme units, 0.5 enzyme units and 1 enzyme unit). You then analyze the plasmid...
and w Two-dimensional gel electrophoresis separates proteins based on a. shape; charge Ob.size; concentration c. concentration;...
and w Two-dimensional gel electrophoresis separates proteins based on a. shape; charge Ob.size; concentration c. concentration; shape O d. size, charge O e. size; shape Refer to the table. Several strains of a bacterium are sequenced to investigate the pan and core genomes. In the table, + denotes presence of the gene and denotes its absence. Gene Gene Gene Gene Gene Strain ! Strain 2 + Strain 3 + Strain 4 + + + + Strain 5 + + What...
e. of ninot0 AOLL 2. A microarray is an orderly arrangement of short strands of DNA attached to a solid surface. Considering SNP microarrays, expression arrays and CNV arrays (array comparative genome hybridization): a. Which type of array(s) will not have intronic sequences attached to the solid surface? Why? b. Which type of array will have a larger proportion of the genome attached to the solid surface? Why? Which type of array would allow one to identify large stretches of...
The following diagram represents a replication bubble associated
with DNA synthesis. Based on this diagram, select all of the
options below that are true.
1 | 2 ---- Quadrants 1 and 4 are associated with lagging strand synthesis Quadrants 1 and 4 are associated with leading strand synthesis Quadrants 1 and 2 are associated with lagging strand synthesis Quadrants 3 and 4 are associated with lagging strand synthesis Synthesis of both daughter strands is completely continuous Telomerase activity is needed...
If you wish to sequence a long strand of DNA in one round of reactions, you should: O A. Decrease the ddNTP/dNTP ratio O B. Increase the ddNTP/dNTP ratio O C. Use a shorter DNA primer O D. Add twice as much primer Based on this figure, the most likely error is: O A. The scientist forgot to add dNTPs to one of the reaction tubes. O O B. <label for="q7_4" id="lq7_4">The scientist did not denature the DNA strands</label> O...
DNA DNA Replication: ONA Because DNA Is the ge m Tumes and heart e ine in process called DNA curs in the nucleus of s acest FS Parent strand Parent strand Newly replicated DNA Newly replicated DNA- SA0 Daughter DNA molecule Daughter DNA molecule Figure 8.2: Overview of DNA replication and illustration of complementary base pairing. DNA must replicate before cell division so that each new daughter cell receives an exact copy of the parent DNA. 1. Replication begins when...
Question 7 estion 7 0.3125 points Suppose that in a Sanger sequencing reaction, sequences are readable for a few dozen nucleotides but then become increasingly difficult to road. It is determined that the chains are terminated to readily. What is the most likely cause? a. Too much of the dNTPs b. Insufficient fluorescent dye Too much fluorescent dye d. Incorrect primer e. Insufficient dNTPs Question of 20 Moving to another question will save this response Question 13 Which statement comparing...
10 please and 7
You isolate plasmid DNA from bacteria (Questions 7-10) 7) A plasmid is an extrachromosomal circular DNA frequently found in prokaryotes. Aside from being smaller, how is it different from the prokaryotic genome? You place equal amounts of plasmid DNA in 4 different tubes and incubate the DNA with increasing amounts of the enzyme topoisomerase I for 1 hour (0 enzyme units 0.25 enzyme units, 0.5 enzyme units and 1 enzyme unit). You then analyze the plasmid...
and w Two-dimensional gel electrophoresis separates proteins based on a. shape; charge Ob.size; concentration c. concentration; shape O d. size, charge O e. size; shape Refer to the table. Several strains of a bacterium are sequenced to investigate the pan and core genomes. In the table, + denotes presence of the gene and denotes its absence. Gene Gene Gene Gene Gene Strain ! Strain 2 + Strain 3 + Strain 4 + + + + Strain 5 + + What...
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