Answer :- nanopore technology.
Whole-genome nanopore sequencing technology. Full genomes of microbials, humans, animals, and plants with long-read nanopore sequencing. Greater similarity between reads increases the assembly of genomes by offering longer, continuous sequences and fewer contigues.
To sequence the whole genome of all the organisms present in a sample, you would use...
QUESTION Which of the following would you use to find a particular sequence within a sample of DNA? O RT-PCR O Western blotting O Northern blotting O Southern blotting QUESTION 7 The Sanger method for DNA sequencing requires 。A. dideoxynucleotides O8. DNA nucleotides OC DNA polymerase O D.A&B O E. A, B, & C .
Assume you are working to sequence the genome of a newly identified alien species. The alien DNA is composed of the same nucleotides are in us and, its genome is 2-3 times larger than that of any terrestrial organism, and this genome is contained within 2 huge linear chromosomes and once smaller circular chromosome. Assume , mechanisms of biology (i.e. transcription, translation, replication, etc. are similar to those acting in terrestrial eukaryotes. That said, here is no reason to believe...
A-E please
10. You inherit a sequencing machine and decide to sequence the genome of an animal. A. (2pts) What animal would you pick? Briefly explain why. B. (6pts) How would you find the protein-coding genes in a new genome sequence? Describe one computational method and one experimental method. C. (4pts) List 4 other DNA elements would you expect to find in a new genome in addition to protein-coding genes? D. (3pts) If you compared the chromosomal organization of dogs,...
Select the steps that occur during whole-genome shotgun sequencing. Check All That Apply Whole genomes are broken down into small DNA fragments. DNA fragments are separated by the process of membrane filtration DNA fragments are cloned into bacterial cells to create a library. Contigs are ordered to create a complete genomic sequence.
Suppose you were using paired-end sequencing with 250 cycles. How much of the genome of a stickleback fish would you expect to sequence at >7X coverage if you used EcoRI/MspI?
Transcriptomics uses following technology: RNAseq Whole genome sequencing Mass Spectrometry Microarray A-All of the above b.1,2 and 4 c. 3 and 4 d. 1 and 4
Question 2
Classical DNA sequencing is a powerful technique. Consider a situation where you first sequence a normal strand of DNA, then sequence a mutated strand. When analysing the results of sequencing reactions, run on a gel, would you be able to distinguish each of the following mutation types, compared to unmutated sequence, using classic "Sanger" sequencing? A silent mutation An insertion of 6 nucleotides A sense mutation Which of the following factors directly breaks phosphodiester bonds? Select all that...
1) You have two human liver cells (A and B) and you hypothesize that the insulin receptor gene in Cell A has a mutation in exon 1 and Cell B contains the wild type sequence. You extract genomic DNA from each of the cells. Of the following, what would be the most efficient (quick, precise and relatively cheap) way to test your hypothesis. a. Isolate protein from both cells, purify the insulin receptor, and determine the amino acid content. b. Sequence the...
find three errors in the pragraph and reconstruct the gel
DI Succuleluujective vi, a 16 points) PCR is a powerful tool used to isolate and make large quantities of a defined DNA fragment from a complex genome. PCR takes advantage of electrophoresis and synthetic oligonucleotides. Its starting material can be as small as a single cell. Because amplification is exponential, a single DNA molecule can be copied into hundreds of copies in a single day. Once a reference DNA sequence...
1. the genes that seem to be the most necessary to maintain, since they are present in the smallest cellular organisms are a. cytoskeletal protein genes b, translation protein genes c. replication protein genes d. transcription protein genes e. DNA repair protein genes 2. Why do cellular organisms generally look very similar when early embryos but different from each other when mature? a. their DNAs have different chemistry b. their RNAs are different lengths c. what genes get turned on...