Homework Answers
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6. Sothern blot will be used to find out a particular sequence in DNA sample
7. Option E is the right answer
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QUESTION Which of the following would you use to find a particular sequence within a sample...
Question 2 Classical DNA sequencing is a powerful technique. Consider a situation where you first sequence...
Question 2
Classical DNA sequencing is a powerful technique. Consider a situation where you first sequence a normal strand of DNA, then sequence a mutated strand. When analysing the results of sequencing reactions, run on a gel, would you be able to distinguish each of the following mutation types, compared to unmutated sequence, using classic "Sanger" sequencing? A silent mutation An insertion of 6 nucleotides A sense mutation Which of the following factors directly breaks phosphodiester bonds? Select all that...
To sequence the whole genome of all the organisms present in a sample, you would use...
To sequence the whole genome of all the organisms present in a sample, you would use PCR, followed by cloning, followed by Sanger sequencing PCR, followed by DGGE, followed by Sanger sequencing CARD-FISH Nanopore technology
Which technique would you use if you wanted to < Identify all the regions of open...
Which technique would you use if you wanted to < Identify all the regions of open chromatin in a particular cell type? Perform a paternity test? Amplify a specific sequence of DNA? [Choose] [Choose] Promoter fusion DNAse-seq Scanning mutagenesis Western blot RNAseq DNA fingerprinting Northern blot Reporter Assay DNAse footprinting ChIP-PCR EMSA Southern blot DNAse protection assay PCR BLAST search Protein fusion ChIP-seq [Choose Compare the global level of RNA expression between two samples? Detect presence of a specific protein...
QUESTION 18 Which of the following enzymes will produce a blunt end (the cut site is...
QUESTION 18 Which of the following enzymes will produce a blunt end (the cut site is indicated by the * in the recognition sequence)? NsiI (ATGCA*T) EcoRV (GAT*ATC) TaqI (T*CGA) EagI (C*GGCCG) QUESTION 19 Which of the following is a functional element of a plasmid? drug-resistance gene origin of replication sequence encoding a restriction endonuclease polylinker sequence QUESTION 20 Next generation sequencing is much more efficient than the Sanger method because: it uses gel electrophoresis to resolve end-labeled strands...
1. Which of the following RNAs can induce gene silencing? tRNA snRNA dsRNA rRNA miRNA 2....
1. Which of the following RNAs can induce gene silencing? tRNA snRNA dsRNA rRNA miRNA 2. Which vehicles are often used for gene therapy to carry a healthy gene? Bacteria Viruses Plastic capsules Both A and B Both A and C 3. A common method in biochemistry to detect and analyze proteins is: Northern blot Southern blot Western blot Eastern blot All of the above 4. The technique used to identify a specific DNA sequence in bacterial colonies is In...
Question 34 Chromosomes can be visualized with fluorescent DNA probes, which complementary bind to chromosomes. What...
Question 34 Chromosomes can be visualized with fluorescent DNA probes, which complementary bind to chromosomes. What is the name of this technique? ADNA fingerprinting BDNA in-situ hybridization (FISH) Sanger sequencing Polymerase chain reaction (PCR) Northern blot Question 35 The cell most often communicates 'opening and closing of the DNA structure via: A methylation on the DNA backbone B. phosphorylation or methylation of mRNA molecules c covalent modifications of histone tails D. 5' capping of genes on DNA E. all of...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...
9. An alteration in the nucleic acid sequence in which a specific restriction endonuclease cuts can...
9. An alteration in the nucleic acid sequence in which a specific restriction endonuclease cuts can be detected by a. ELISA b. flow cytometry c. RFLP d. FISH 10. Which of the following techniques enables the identification of a particular sequence of nucleic acid on a cell or tissue? a. northern blot b. western Blot c. FISH d. southern blot 11. The polymerase chain reaction is used to a. convert RNA back into DNA b. amplify a target nucleic acid...
.Select the probe sequence that will hybridize to the following nucleic acid sequence: C G A...
.Select the probe sequence that will hybridize to the following nucleic acid sequence: C G A T A T T G T C A. T A G T A C A A G A B. C G A T A T T G T C C. G T C A A G A C C T D G C T A T A A C A G Select the strand of RNA that is complementary to this single strand of...
6) Many scientists doubted that shotgun sequencing would work, even with the smallest genomes, because: a....
6) Many scientists doubted that shotgun sequencing would work, even with the smallest genomes, because: a. There would not be overlaps between the different mini-sequences. b. Computers would be unable to handle the huge amount of data generated by a shotgun sequencing project. c. Small prokaryotic genomes contain large amounts of repetitive DNA. d. No method existed for breaking genomic DNA into random fragments. 7) In a PCR-RFLP assay, enzyme digestion revealed three bands (152+187+462bp) in AA animals, four bands...
Question 2
Classical DNA sequencing is a powerful technique. Consider a situation where you first sequence a normal strand of DNA, then sequence a mutated strand. When analysing the results of sequencing reactions, run on a gel, would you be able to distinguish each of the following mutation types, compared to unmutated sequence, using classic "Sanger" sequencing? A silent mutation An insertion of 6 nucleotides A sense mutation Which of the following factors directly breaks phosphodiester bonds? Select all that...
To sequence the whole genome of all the organisms present in a sample, you would use PCR, followed by cloning, followed by Sanger sequencing PCR, followed by DGGE, followed by Sanger sequencing CARD-FISH Nanopore technology
Which technique would you use if you wanted to < Identify all the regions of open chromatin in a particular cell type? Perform a paternity test? Amplify a specific sequence of DNA? [Choose] [Choose] Promoter fusion DNAse-seq Scanning mutagenesis Western blot RNAseq DNA fingerprinting Northern blot Reporter Assay DNAse footprinting ChIP-PCR EMSA Southern blot DNAse protection assay PCR BLAST search Protein fusion ChIP-seq [Choose Compare the global level of RNA expression between two samples? Detect presence of a specific protein...
Question 34 Chromosomes can be visualized with fluorescent DNA probes, which complementary bind to chromosomes. What is the name of this technique? ADNA fingerprinting BDNA in-situ hybridization (FISH) Sanger sequencing Polymerase chain reaction (PCR) Northern blot Question 35 The cell most often communicates 'opening and closing of the DNA structure via: A methylation on the DNA backbone B. phosphorylation or methylation of mRNA molecules c covalent modifications of histone tails D. 5' capping of genes on DNA E. all of...
Carolina Savirana Craz 3/12/20 GECC-Polymerase Chain Reaction 1. What is the purpose of the polymerase chain reaction? a. To repair damaged DNA b. To make copies of entire chromosomes c. To make copies of specific regions of DNA d. To prepare cells for cell division 2. The polymerase chain reaction is most comparable to what cellular process? a. Mitosis b. Replication c. Transcription d. Translation 3. When enzymes are elongating (building) a newly synthesized DNA strand in PCR, new nucleotides...
6) Many scientists doubted that shotgun sequencing would work, even with the smallest genomes, because: a. There would not be overlaps between the different mini-sequences. b. Computers would be unable to handle the huge amount of data generated by a shotgun sequencing project. c. Small prokaryotic genomes contain large amounts of repetitive DNA. d. No method existed for breaking genomic DNA into random fragments. 7) In a PCR-RFLP assay, enzyme digestion revealed three bands (152+187+462bp) in AA animals, four bands...
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