Question

1. Which of the following RNAs can induce gene silencing?

1. tRNA
2. snRNA
3. dsRNA
4. rRNA
5. miRNA

2. Which vehicles are often used for gene therapy to carry a healthy gene?

1. Bacteria
2. Viruses
3. Plastic capsules
4. Both A and B
5. Both A and C

3. A common method in biochemistry to detect and analyze proteins is:

1. Northern blot
2. Southern blot
3. Western blot
4. Eastern blot
5. All of the above

4. The technique used to identify a specific DNA sequence in bacterial colonies is

1. In situ hybridization
2. In vivo hybridization
3. Colony hybridization
4. Southern hybridization
5. Northern hybridization

5. The enzyme used in the formation of cDNA from mRNA is

1. Helicase
2. Topoisomerase
3. DNA polymerase
4. RNA polymerase
5. Reverse transcriptase

Answer 1

1. Correct answer is E. miRNA

miRNA and siRNA are used for gene silencing.

2. Correct answer is B. viruses

Viruses used to infect the target cell and transfer the desired gene.

3. Correct answer is C. Western blot

Northern blot - Detection of RNA

Southern blot - Detection of DNA

Eastern blot - Detection of post translational modifications of proteins in addition of lipid, glycoconjugates and phosphates

4. Correct answer is C. Colony hybridization

5. Correct answer is E. Reverse transcriptase