Question

"R" group polarity nonpolar, polar, or polar with H- bonds.
1. choose any two know amino acids with similar Rf and based on the size and polarity of their side chains, give an explanation for why their retention factors are similar.
2. choose any two known amino acids with very different Rf values and based on the size and polarity of their side chains, give an explanation for why their retention factors are dissimilar.
3. Explain why lysines Rf is lower than that of leucine, even thought they both have the same number of carbon atoms in their side chains.
4. when you supplied the beaker with solvent, you were cautioned that the solvent level should be below the sample spotted on the chromatography paper. why?

Distance Traveled by Amino Acid "R"group Polarity поnpolar, polar, or polar with H- bonds Color after ninhydrin spray liant Amino Acids Name Macae Hilly Date 10-30-19 Section_22 Partner Miyayla Morris Paper Chromatography (Ipt for cach blank /50) Size of "R" Distance Group Amino Acid Traveled by Rf small Mobile Phase medium, large Senne s 51.0mm 99. mm.515 large Porple Leucinch 75.0mm 99.0mm .758 small purple Tyrosine Y 63.0mm 100. Omm 6300 medium Dorple Dark Lysine K 43.0mm 99.0mm.434 large purple Tryptophan 65.0mm 99.0mm.657 medum purple Glycine G51.0mm 99.0mm.515 large Purple Alaninc A 52.0mm loo.mm.5200 medium forple Aspartic Acid 46mm 100.0mm.6400 large Blue Unknown 45.0mm 102.0mm.4412 Unknown code 15 Unknown identity (J0 pre-No wow.code Upis) In complete sentences on the back of this page or on a separate piece of paper answer the following questions (5 pts each) x Blue 1. Choose any two known amino acids with similar and based on the size and polarity of their side chains give an explanation for why their retention factors are similar. 2. Choose any two known amino acids with very different Rivalues and based on the size and polarity of their side chains, give an explanation for why their retention factors are dissimilar. 3. Explain why lysine's Rris lower than that of leucine, even though they both have the same number of carbon atoms in their side chains. 4. When you supplied the beaker with solvent, you were cautioned that the solvent level should be below the samples spotted on the chromatography paper. Why? 34

Answer 1

I5OLencine 1. A ame Leucine will nae RE valme since thy howe similan R-gup similan isomen oA one Oanothar. alanin Fom the gien data gycine A adanealso have similan R valne since they are vermy 8inmmlan bo o ne anotnan witn nuspect to funchional 0mp onty on carbon extha fon alakin A Lainine ond ysin wil have oliffrunt 2. RF ne nce dt alanine has CH as its ica chain A ine har Langen side chaine and -NH as functonal gonp which wiu make it mor opared to alanine polan one addibion functional aop 3. Lysine has e NH2 gonp abachol to its sidle chain which makes it moe polan ompared to Leucine which oloesnot have additional Aunctonl Hence ySin has Re is owen thon Laueine u. S0lvent Level shonldl be belos the Spot becane othenwise tne sowent may dissowe tne spots A make tem come ont of tne TLC plate into te sowens nather tuan maving tuem upwand tne TLC on