Question

A biochemist studying the activity of the enzyme triose phosphate isomerase (TPI) in a species of Drosophila wants to know if the mean activity of this enzyme is the same at ??=5 and ??=8.

Enzyme activity (?????)
?? = 5	11.1	10.0	13.3	10.5	11.3
?? = 8	12.0	15.3	15.1	15.0	13.2

a. Given the above data and assuming enzyme activities are normally distributed, determine if the mean activities of TPI are significantly different at these two pH levels.

b. Give the 95% confidence interval for ?5−?8.

Answer 1

(Page No Date PH=5 X, 111 10 13.3 10.5 11.3 5612 PH=8 X2 12 15.3 15.1 15 13.2 70.6 x? 1 23.21 100 176.89 110.25 127.69 638.04 x² 144 ce 234.09 228.01 225 174.29 1005.34 X, - Exi. 56.2 = 11:24 72= E 70.6 - 14.12 5 Exi2_n, CF,)? hool 638.04-5(11.242 5 -1 -T si = 1.2601 S2 = 1 Exo? - 02 (8212 V n2-) 1005.34-5(14.122 S7 S2 = 1.4549 Sp- en,-1) 5,² + (12-1) 5² not n2-2 1 (5-1) ( 1.26012+(5-1) (1.45692 - 5+5-2 1.3610

(Page No (Dato Test Ho: el = ll vis Hiiel & ele Test stabstic, t= *,-X2 11:24 - 14.12 spt the 1.3610 x } } - 2.88 0.8607 = -3.3461 of = nitaz-z = 5+5-2 =10-2 = 8 D-value = PCIe), ) =2.pl tas-3-3461) Drvalue = 0.0101 Decision Rue p-valtio=00101<Q=0.05 Reject Ho. The mean activities of TPſ are signi Bcantly different at these two PH levels. 6 95% ca (8,-F2) t tx2, nt & Sp 1 to (11.24-1612) I to.05/2,8 X 1-36 -2.88 12.30 x 0.8607 -2.88 I 1.9796 (-2.88-1.9796, -2.88+ 1.9796) (-4.8596 -0.goou) -4.8596L els lls <-o.goou