Question

30. Describe the difference between transition state and intermediate using energy diagrams. 31. For enzymes in which the slowest (rate-limiting) step is the reaction k2 ES P Km becomes equivalent to: A. Kcat- B. the [S], where Vo Vmax C. the dissociation constant, Ka, for the ES complex. D. the maximal velocity. E. the turnover number. 33. Draw a schematic for sequential mechanism 32. An enzyme "ABase" catalyzes the following reaction: A B. You found that for [E] 4 nM, Vmax measured was 1.6 HMS1. What is the Kcat for ABase? (include the unit). with random attachment.

Answer 1

30.

Transition state The state at which the berwation bdnd bouds and ake plae Intermediate It is an unstable mdecule or Stable molecule that is n the between t reactaut aud product energy iaga m is shomm belon: The Trausition states Energt Tntermediate eactiou coordinate