Question

6. The recipe for cell culture media is: 10 % serum, 1x antibiotics and 2 mM Glutamine in basal media. You are given the following stock solutions: • Basal media • Serum 100% • Antibiotics 100x solution Glutamine 200 mm How will you prepare the 500 mls of media? (2 point) (Note: A 100X solution is 100 times more concentrated than the concentration required in the final solution. For example if a 250 mM NaCl stock solution is said to be 100X it means that the final concentration of NaCl in the solution has to be 2.5 mM. So a 100X solution has to be diluted 100 times to make the final solution.)
please help solve this and show all work clearly, thanks

Answer 1

6. The calculations can be made using the dilution law

N₁V₁ = N₂V₂

N₁ = Initial concentration of the component

V₁ = Initial volume of the solution

N₂ = Final concentration of the component

V₂ = Final volume of the solution

Given that the recipe for the cell culture is

10 % serum

1x antibiotics

2 mM Glutamine

These are the final concentrations of serum, antibodies and glutamine in the final 500 mL cell culture media.

Given stock solution are

serum - 100 %

antibiotics - 100x

glutamine - 200 mM

Now we should calculate the initial volumes of stocks to prepare 500mL cell culture media

1. Serum

N₁V₁ = N₂V₂

100 % x V₁ = 10 % x 500 mL

V₁ = 5000 / 100 = 50 mL of serum

2. antibiotics

N₁V₁ = N₂V₂

(100x) x V₁ = (1x) x 500 mL

V₁ = 500/100 = 5 mL of antibiotics

3. Glutamine

N₁V₁ = N₂V₂

200 mM x V₁ = 2 mM x 500 mL

V₁ = 1000 / 200 = 5 mL

4. Basal media

These three are taken in basal media. Hence the cell culture media should be make up to 500 mL with basal media.

Thus the volume of basal media = Volume of final solution - Volume of other components

The volume of basal media = 500 - (50 + 5 + 5) = 500 - 60 = 440 mL

Thus we can prepare 500 mL of cell culture media by taking 440 mL of basal media, 50 mL of stock serum, 5 mL of stock antibiotics and 5 mL of stock glutamine