Question

Please answer 2 and 3:

2. Differential scanning calorimetry can be used to study the stability of a protein or polymer. Which of the following are true about this technique? a) the enthalpy of unfolding corresponds to the area under the curve b) the more stable a protein or polymer, the higher the melting temperature c) the Gibbs free energy determines of melting is spontaneous d) plotting the function of temperature determines Ink e) adding urea or acid to the sample will lower the Tm of a protein 3. Which of the following is true about the standard Gibbs energy, AGO? a) it is related to the total (universe) entropy change b) AG° = 0 at equilibrium c) TASO = AH° d) it is --RT In K e) it is equal to the total work minus PV work

Answer 1

2. Differential scanning calorimetry is an analysis technique to characterize the stability of a protein. In normal condition, the native form I folded) and denarared renfolded) conformations are at equilibrium when thermal energy is provided, the protein unfolds. The more stability, greater transition midpoint (Tm) is obtained. Hence, option. (b) is correct Gibbs' from correct. free energy is a measure of unavailable work the system. Hence, was the option (d) is However, at equilibrium, auto is also Furthermore, au=- Rienk is also true is the equilibrium constant correct if k