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3. A student used a medicine dropper, instead of a toothpick, to spot the standard 4-cation solutions on the chromatographic


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If medicine dropper is used instead of toothpick to spot the standard 4 -cation solutions on the chromatographic paper. The resulting original spots were each 1.0 cm in diameter.

The spot size should not be larger than 1 mm in diameter.

The effect on the results due to this spot diameter leads to poor separation. This is due to the over large spot that cause overlapping of other component spots with similar retention factor (Rf) values.

If this happens then the components cannot be resolved.

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