Question

Describe the following conditions:

(a) The following is observed in the analysis of a potential suspect. At the crime scene DNA from a blood stain NOT linked to the victim, is shown to be heterozygous for D1S80, with bands corresponding to 18 and 24 repeats. Suspect 1 is shown to be homozygous for D1S80 and the band corresponds to approximately 430 bp. Can this suspect be eliminated? How many repeats of D1S80 are present in this individual’s genome?

(b) Suspect 2 is heterozygous for D1S80, with bands of approximately 430 bp and 530 bp. Can this suspect be eliminated? How many repeats of D1S80 are present in this individual’s genome?

Answer 1

Charecteristics of the D1S80 locus useful for genetic linkage studies,genetic polymorphism used as markers for personal identification,study of inheritance, population genetics and genetic disorders..unmatched DNA does not usually take it for functional importance..variable number tandem repeats (VNTRs) can lead to errors in DNA..The variation means that inherit different alleles from each parent,making more people heterozygous..

The most common alleles are comprised of 18to 24 repeat units..genetic differences between individual identifying every possible difference in their DNA..It need more cost and time..when using DNA identification,it is must to identify region of DNA that have high variability between individual..when each VNTRs and DNA simultaneously compared VNTR locus two people alleles may match by chance sometime.. Because the size difference regulates focus in the D1S80 locus on the order of more pairs..VNTR analysis done based on the STR analysis and for DNA identification...Difference between one locus instead of the 20usually used for criminal forensic data..