Homework Answers
It is an initial-rate equation for a single-substrate noncooperative enzyme-catalyzed reaction relating the initial velocity to the initial substrate concentration.
It takes the form of an equation relating reaction velocity to substrate concentration for a system where a substrate S binds reversibly to an enzyme E to form an enzyme-substrate complex ES, which then reacts irreversibly to generate a product P and to regenerate the free enzyme E. This system can be represented schematically as follows:
![Ets Esz Erp The michaelis-menten eanation for this system is • Ve Vmax [s] . Kms+ [s] .. Vmax = represents the maximum veloci](http://img.homeworklib.com/questions/1bed5ab0-2aae-11ea-b1f8-190f3c0dcf98.png?x-oss-process=image/resize,w_560)
This is a plot of the Michaelis-Menten equation’s predicted reaction velocity as a function of substrate concentration, with the significance of the kinetic parameters Vmax and Km graphically depicted.
![Recetim velocity v .Vmax - - - - - Vmax/2 .. substrate concentrations [5]](http://img.homeworklib.com/questions/1caa2bc0-2aae-11ea-8ad8-894eae6a4d8e.png?x-oss-process=image/resize,w_560)
Michaelis-menten equation does not apply to enzymes that display sigmoidal V0 vs. [S] curves, but only to those who giving hyperbolic kinetic curve.
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1. (15 points) Give the Michaelis-Menten equation and define each term in it (no need to derive the equation). Does...
(5) The Michaelis-Menten equation describes the following simplistic pathway Km (a) State each of the assumptions...
(5) The Michaelis-Menten equation describes the following simplistic pathway Km (a) State each of the assumptions on which the Michaelis-Menten equation is based. (b) Derive the rate law for this process
4. Basic concepts of Michaelis-Menten kinetics. The Michaelis-Menten equation is expression of the relationship between the...
4. Basic concepts of Michaelis-Menten kinetics. The Michaelis-Menten equation is expression of the relationship between the initial velocity, Vo, of an enzymatic reaction and substrate concentration, [S]. There are three conditions that are useful for simplifying the Michaelis-Menten equation: [S] <<Km; [S] = Km; [S] >> Km. Match each condition with the statement(s) that describe it. TV, Vmox[S] Vo =Vmax m . V Vo - Vmax [S] Km +[S] V. (um/min) max [S] (mm) (a) Doubling [S] will almost double...
7. a) In an enzyme catalyzed reaction which follows the Michaelis-Menten kinetics. The substrate ...
7. a) In an enzyme catalyzed reaction which follows the Michaelis-Menten kinetics. The substrate concentration (Km, Michaelis constant) needed to reach 50% of the maximum reaction velocity (Vmax) is 20 μΜ. What substrate concentration is required to obtain at least 75% of the maximum reaction velocity? Show the work to get full points. (5 points) b) You want to load 10 μg of protein in 15 μL into one of the 10% polyacrylamide gel well. The protein needs to be...
CHEM 304: Biochemistry Name: 5, What type of plot is given below? What does each intercept...
CHEM 304: Biochemistry Name: 5, What type of plot is given below? What does each intercept represent? What type of inhibition is represented in the following plot? Explain why. (12 pts [1] Increasing No inhibitor 0 [Sl 6. Write out the equation that describes the mechanism for enzyme action used as a model by Michaelis and Menten. List the important assumptions used by Michaelis and Menten to derive a rate equation for this reaction.(10 pts)
The Michaelis-Menten equation models the hyperbolic relationship between (S) and the initial reaction rate V, for...
The Michaelis-Menten equation models the hyperbolic relationship between (S) and the initial reaction rate V, for an enzyme-catalyzed, single-substrate reaction E+ S E S E+P. The model can be more readily understood when comparing three conditions: [S] << Km, [S] = Km, and [S] >> K. Match each statement with the condition that it describes. Note that "rate" refers to initial velocity Vwhere steady state conditions are assumed. [Exotal) refers to the total enzyme concentration and [Etre) refers to the...
The Michaelis-Menten equation models the hyperbolic relationship between [S] and the initial reaction rate (V0) for...
The Michaelis-Menten equation models the hyperbolic relationship
between [S] and the initial reaction rate (V0) for an enzyme
catalyzed, single substrate reaction: E S ES E P. The model can be
more readily understood when comparing three conditions: [S]Km.
Match each statement with the condition that it describes. Note:
\"Rate\" refers to initial velocity (V0) where steady state
conditions are assumed; [Etotal] refers to the total enzyme
concentration, and [Efree] refers to the concentration of free
enzyme.
categories: [S]<<Km, [S]=Km,...
Please mark all that apply, there may be more than one answer for each question, thank...
Please mark all that apply, there may be more than one answer for each question, thank you!: Under which of the following conditions would the Michaelis-Menten equation not hold true? [ ] [S] is constantly changing [ ] [E] is constantly changing [ ] [S] and [P] are constant [ ] [P] is constantly changing Why is it difficult to determine the Vmax from a Michaelis-Menten graph? [ ] It is a complex calculation [ ] Cannot plot a curve...
[15 points] Give careful definitions for each term (in the context of computer operating systems): Process...
[15 points] Give careful definitions for each term (in the context of computer operating systems): Process Control Block Blocking send and Non-blocking send Race condition Critical-Section problem Deadlock
1. Falling Ball with Air Resistance (15 points) A round bal is dropped from a height for which ai...
1. Falling Ball with Air Resistance (15 points) A round bal is dropped from a height for which air resistance is not negligible by the time the ball hits the ground A. On what dimensional parameters does the ball's impact speed depend? Define al symbols and give their dimensions B. Show at least three different ways that the impact speed may be made dimensionless C. On what dimensionless parameter(s) does the dimensionless speed depend? 2. Laminar Jet Toward Plate (15...
In lab Review Lecture 8 Name 2 points for each answer Lab, morning noon afternoon Define...
In lab Review Lecture 8 Name 2 points for each answer Lab, morning noon afternoon Define an exothermic reaction. 1. 2. Does breaking a chemical bond require energy or give off energy? Is it endothermic or exothermic? Define the term entropy 3. 4. Write the equilibrium equation for the following reaction NH,NO, (s) N,O (g) + 2 H,O (g) For the reaction in question 4, what would be the change in the concentrations of reactants 5. and products if %...
(5) The Michaelis-Menten equation describes the following simplistic pathway Km (a) State each of the assumptions on which the Michaelis-Menten equation is based. (b) Derive the rate law for this process
4. Basic concepts of Michaelis-Menten kinetics. The Michaelis-Menten equation is expression of the relationship between the initial velocity, Vo, of an enzymatic reaction and substrate concentration, [S]. There are three conditions that are useful for simplifying the Michaelis-Menten equation: [S] <<Km; [S] = Km; [S] >> Km. Match each condition with the statement(s) that describe it. TV, Vmox[S] Vo =Vmax m . V Vo - Vmax [S] Km +[S] V. (um/min) max [S] (mm) (a) Doubling [S] will almost double...
7. a) In an enzyme catalyzed reaction which follows the Michaelis-Menten kinetics. The substrate concentration (Km, Michaelis constant) needed to reach 50% of the maximum reaction velocity (Vmax) is 20 μΜ. What substrate concentration is required to obtain at least 75% of the maximum reaction velocity? Show the work to get full points. (5 points) b) You want to load 10 μg of protein in 15 μL into one of the 10% polyacrylamide gel well. The protein needs to be...
CHEM 304: Biochemistry Name: 5, What type of plot is given below? What does each intercept represent? What type of inhibition is represented in the following plot? Explain why. (12 pts [1] Increasing No inhibitor 0 [Sl 6. Write out the equation that describes the mechanism for enzyme action used as a model by Michaelis and Menten. List the important assumptions used by Michaelis and Menten to derive a rate equation for this reaction.(10 pts)
The Michaelis-Menten equation models the hyperbolic relationship between (S) and the initial reaction rate V, for an enzyme-catalyzed, single-substrate reaction E+ S E S E+P. The model can be more readily understood when comparing three conditions: [S] << Km, [S] = Km, and [S] >> K. Match each statement with the condition that it describes. Note that "rate" refers to initial velocity Vwhere steady state conditions are assumed. [Exotal) refers to the total enzyme concentration and [Etre) refers to the...
The Michaelis-Menten equation models the hyperbolic relationship
between [S] and the initial reaction rate (V0) for an enzyme
catalyzed, single substrate reaction: E S ES E P. The model can be
more readily understood when comparing three conditions: [S]Km.
Match each statement with the condition that it describes. Note:
\"Rate\" refers to initial velocity (V0) where steady state
conditions are assumed; [Etotal] refers to the total enzyme
concentration, and [Efree] refers to the concentration of free
enzyme.
categories: [S]<<Km, [S]=Km,...
1. Falling Ball with Air Resistance (15 points) A round bal is dropped from a height for which air resistance is not negligible by the time the ball hits the ground A. On what dimensional parameters does the ball's impact speed depend? Define al symbols and give their dimensions B. Show at least three different ways that the impact speed may be made dimensionless C. On what dimensionless parameter(s) does the dimensionless speed depend? 2. Laminar Jet Toward Plate (15...
In lab Review Lecture 8 Name 2 points for each answer Lab, morning noon afternoon Define an exothermic reaction. 1. 2. Does breaking a chemical bond require energy or give off energy? Is it endothermic or exothermic? Define the term entropy 3. 4. Write the equilibrium equation for the following reaction NH,NO, (s) N,O (g) + 2 H,O (g) For the reaction in question 4, what would be the change in the concentrations of reactants 5. and products if %...
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