Phenol-Sulfuric Acid Method for Carbohydrate Analysis Which carbohydrate groups does the method analyze? What is the...
Phenol-Sulfuric Acid Method for Carbohydrate Analysis
- Which carbohydrate groups does the method analyze?
- What is the principle of the method? Is a standard curve needed? Why?
- When do you add phenol reagent, before or after acid addition? What is the role of the phenol reagent?
- What dilution scheme would you follow for a regular soft drink? For a light beer? Explain your answer.
Homework Answers
ANSWER
Phenol-Sulfuric Acid Method is most reliable method for carbohydrate estimation. it is mostly use in measuring neutral sugar content in oligosaccharides, proteoglycanes, glycoproteins and glycolipids.
In hot acidic medium, glucose is dehydrated to hydroxymethyl
furfural. This form yellow-brown coloured product with phenol and
its absorption maximum at 290nm. 
Here 5% phenol is being used as regent and for the method while 90-95% sulfuric acid is used.
Yes a standard curve needed, The phenol sulfuric acid methode is carried out by preparing a set of solution with known glucose concentration and mixing them with the phenol- sulfuric acid regent. A standard curve can be made and the concentration of unknown sugar sample can be derived from the standard curve.
In the test of Color, Color is observed after addition of sulfuric acid and phenol is intensify the color.
Soft drink Carbonated water constitute up to 94% of soft drink. CO2( uniquely suitable gas for soft drink and easy to liquefy) added to special sparkle and bite to the beverage and also act as a mild preservative. And the second main ingredient is sugar, which makes up to 7-12%.
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Phenol-Sulfuric Acid Method for Carbohydrate Analysis Which carbohydrate groups does the method analyze? What is the...
QUESTIONS 1. What is the purpose of the sulfuric acid (yes, it's a catalyst, but why...
QUESTIONS 1. What is the purpose of the sulfuric acid (yes, it's a catalyst, but why is it a catalyst? What is it doing that makes the reaction possible? Would the reaction just go slower without it, or would the reaction not go at all? Why?) 2. If isobutyl alcohol (2 methyl propanol) is used instead of tert butyl alcohol, a mixture of products is obtained. One the products is the same as the product obtained with the tert butyl...
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2) What chemical reaction does the titrant contribute to this experiment? CHEM 1A Experiment #8: Measuring the Vit...
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What chemical reaction does the titrant contribute to this
experiment?
CHEM 1A Experiment #8: Measuring the Vitamin C Content of Emergen-C™ Objectives: Students will learn how to use a burette. Students will learn how to use the iodine starch indicator system to monitor oxidation/reduction reactions. • Students will learn how to conduct a titration to determine the amount of analyte in an unknown solution. • Students will learn about ascorbic acid's role as a biological reducing agent. Introduction: Vitamin...
1. What is the definition of an 'equivalence point' in an acid/base titration? (1 point) 2....
1. What is the definition of an 'equivalence point' in an acid/base titration? (1 point) 2. In part one of the experiment, you will prepare the acid solutions being titrated from a stock solution. Describe how you will accurately prepare 10.00 mL of 0.100 M HCl solution using a 1.00 M HCl stock solution. In your response to this question, be very specific about the quantities of stock solution and deionized water to be used in the dilution and the...
1) What chemical is the titrant in this experiment? What chemical is the analyte in this experiment? Experi...
1) What chemical is the titrant in this experiment? What
chemical is the analyte in this
experiment?
Experiment #8: Measuring the Vitamin C Content of Emergen-C™ Objectives: • Students will learn how to use a burette. • Students will learn how to use the iodine starch indicator system to monitor oxidation/reduction reactions. Students will learn how to conduct a titration to determine the amount of analyte in an unknown solution. • Students will learn about ascorbic acid's role as a...
3. If 15.0 mL of 0.125 M phosphoric acid is titrated with 0.100 M NaOH, what...
3. If 15.0 mL of 0.125 M phosphoric acid is titrated with 0.100 M NaOH, what volume of the titrant (in mL) must be added to completely neutralize the acid? Show all of your work (including the chemical equation). (1 point) Post-lab Questions: Experiment #9: Acid-Base Titrations Student Learning Objectives : Students will gain practice with the accurate preparation of solutions. Students will perform acid-base titrations and prepare titration curves. Students will identify strong and weak acids by the shapes...
need help with the boxed bullet point. writing the chemical reaction scheme for the experiment. Synthesis of an A...
need help with the boxed bullet point. writing the chemical
reaction scheme for the experiment.
Synthesis of an Alkyl Halide: A Nucleophilic Substitution Reaction, Part I Pre-lab Assignment for Synthesis of an Alkyl Halide: A Nucleophilic Substitution Reaction, Part 1 1. From The Organic Chem Lab Survival Manual ( edition) by Zubrick • Review pages 201-204: Standard Reflux. • Review pages 127-140: Extraction • Review pages 164-189: Distillation, Simple Distillation. The Distillation Example, and The Distillation Mistake. 2. Read this...
please identify the unknown and write a derivative Unknown compound 3 Clear liquid Physcial Properties Solubility...
please identify the unknown and write a derivative
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It's a weak acid strong base titration Experiment 4: Identification of an unknown acid by titration...
It's a weak acid strong base titration
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I need to write a conclusion for this lab. Based off these results what kinds of...
I need to write a conclusion for this lab. Based off these
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S OY Orre tes. A, B. Color, pH, Specific Gravity, and Electrolytes "Normal Urine Specimen" Test A. Color I clear "Abnormal Urine Specimen" slightly cloudy PH 2 1.10 gime! Specific gravity 0.70 glume - B. Electrolytes (Indicate absent - present +, strongly present ++) nornal Nat CI SO,2- PO,- 219 C-F. Glucose, Ketone Bodies, Protein, and Urobilinogen "Normal Urine Specimen"...
QUESTIONS 1. What is the purpose of the sulfuric acid (yes, it's a catalyst, but why is it a catalyst? What is it doing that makes the reaction possible? Would the reaction just go slower without it, or would the reaction not go at all? Why?) 2. If isobutyl alcohol (2 methyl propanol) is used instead of tert butyl alcohol, a mixture of products is obtained. One the products is the same as the product obtained with the tert butyl...
2)
What chemical reaction does the titrant contribute to this
experiment?
CHEM 1A Experiment #8: Measuring the Vitamin C Content of Emergen-C™ Objectives: Students will learn how to use a burette. Students will learn how to use the iodine starch indicator system to monitor oxidation/reduction reactions. • Students will learn how to conduct a titration to determine the amount of analyte in an unknown solution. • Students will learn about ascorbic acid's role as a biological reducing agent. Introduction: Vitamin...
1. What is the definition of an 'equivalence point' in an acid/base titration? (1 point) 2. In part one of the experiment, you will prepare the acid solutions being titrated from a stock solution. Describe how you will accurately prepare 10.00 mL of 0.100 M HCl solution using a 1.00 M HCl stock solution. In your response to this question, be very specific about the quantities of stock solution and deionized water to be used in the dilution and the...
1) What chemical is the titrant in this experiment? What
chemical is the analyte in this
experiment?
Experiment #8: Measuring the Vitamin C Content of Emergen-C™ Objectives: • Students will learn how to use a burette. • Students will learn how to use the iodine starch indicator system to monitor oxidation/reduction reactions. Students will learn how to conduct a titration to determine the amount of analyte in an unknown solution. • Students will learn about ascorbic acid's role as a...
3. If 15.0 mL of 0.125 M phosphoric acid is titrated with 0.100 M NaOH, what volume of the titrant (in mL) must be added to completely neutralize the acid? Show all of your work (including the chemical equation). (1 point) Post-lab Questions: Experiment #9: Acid-Base Titrations Student Learning Objectives : Students will gain practice with the accurate preparation of solutions. Students will perform acid-base titrations and prepare titration curves. Students will identify strong and weak acids by the shapes...
need help with the boxed bullet point. writing the chemical
reaction scheme for the experiment.
Synthesis of an Alkyl Halide: A Nucleophilic Substitution Reaction, Part I Pre-lab Assignment for Synthesis of an Alkyl Halide: A Nucleophilic Substitution Reaction, Part 1 1. From The Organic Chem Lab Survival Manual ( edition) by Zubrick • Review pages 201-204: Standard Reflux. • Review pages 127-140: Extraction • Review pages 164-189: Distillation, Simple Distillation. The Distillation Example, and The Distillation Mistake. 2. Read this...
please identify the unknown and write a derivative
Unknown compound 3 Clear liquid Physcial Properties Solubility Dissolve in ethyl ether Not dissolved in water Boiling point 77 IR spectrum Transmitance 3000 1000 2000 Wavenumber cm-1) Classification Positive test in Alkaline Iron (III) Hydroxamate test test CLASSIFICATION TESTS These tests must be done together with known AND FOLLOW PROCEDURE IN YOUR TEXT CARBOXYLIC ACIDS are detected by teating aqueous solutions with limus or pH paper. Also, disolve In NaHCO with bubbles...
It's a weak acid strong base titration
Experiment 4: Identification of an unknown acid by titration Page 2 of 15 Background In this experiment, you will use both qualitative and quantitative properties to determine an unknown acid's identity and concentration. To do this analysis, you will perform a titration of your unknown acid sample-specifically a potentiometric titration where you use a pH meter and record pH values during the titration, combined with a visual titration using a color indi- cator...
I need to write a conclusion for this lab. Based off these
results what kinds of conclusions can be drawn?
S OY Orre tes. A, B. Color, pH, Specific Gravity, and Electrolytes "Normal Urine Specimen" Test A. Color I clear "Abnormal Urine Specimen" slightly cloudy PH 2 1.10 gime! Specific gravity 0.70 glume - B. Electrolytes (Indicate absent - present +, strongly present ++) nornal Nat CI SO,2- PO,- 219 C-F. Glucose, Ketone Bodies, Protein, and Urobilinogen "Normal Urine Specimen"...
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