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The column chromatography procedure for the separation of a polar and a non- polar compound calls...

  1. The column chromatography procedure for the separation of a polar and a non- polar compound calls for sequential elution with the following solvents: hexane, 70:30 hexane : acetone, acetone and 80:20 acetone : methanol.
    Why it is important to follow this solvent order?

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It is important to follow this solvent order as it allows the compounds to elute from the column according to their polarity. For example: hexane will allow non polar compounds to come out of the column followed by some mid polar compounds which come out from the column by increasing the polarity to  70:30 hexane : acetone. the polar compounds will be out from the column by using acetone as mobile phase. Very polar compounds will only be eluted by using 80:20 acetone : methanol.

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