Question

The hint was: might need to do a serial dilution as outr pipettes cannit dispense less than 2 microL.

SHOW ALL WORK/EXPLAIN how would this be made

on quiz it was / 10mm How would you make 100 ML of LoomM glucose solution from a Mglucose STOCK Solution? IM > 1000 MM ciz 1000 m M C2 = 100mm Vix va = 100 ML 1000 x = 10 (100) x = 10ML lif 10mm .. X = LML our pipettes cannot dispense less than 2 ur

Answer 1

Concentrati on of standard stocksoluti on, M = 1M (1) For 100 Lof 100 mM solution Concentrati on of solution, M = 100 mM volume of solution, = 100 /L required volume is calculated as follows 100 mMx 100L 103M X 1 M 1 mM 10 L Therefore, 10L of stock solution should be put in volumetric flask and add water until the volume reaches to 100L (2) For 100 Lof 10 mM solution Concentrati on of solution, M = 10 mM volume of solution, = 100 L required volume is calculated as follows M MV Му, 10 mMx100L 103 M X 1 mM 1 M 1L Therefore, 1Lof stock solution should be put in volumetric flask and add water until the volume reaches to 100 pL