Question

discuss the std curve and report on the accuracy of each sample

Sample Table Report 06/11/2019 O1.12 33 PM File Name: CUVProbe-DataMethodsICell BioProteins-Bradford Std Curve 190611 125913 pho Sample Graph 1321 F3 1000 0.500 0.000 2 6 Sequence No. Sample Table e.7YS N=3 595 nm Sample ID fa Туре Fr Conc Comments I1 0.745 0.891 Unknown 2 0.244 0.292 4 x4 12 0.505 1.460 0.8631 14 0.562 4 144 0 683 xI 571 0401 y .67 0.228 X4 1b Unknown Unknown Unknown Unknown Unknown Unknown Unknown Unknown 3 /2 1c 0.422 2a 1.220 2 2c 2tb 0.721 6 Y0.461 0571 0.3352. 0.191 3a 3b N3 S710.764 0.764 3c 10 Page 1/1 u) uo

the samples are just protein 1,2 and 3. and each one diluted as shown there .

Answer 1

Answer:-

1. The above graph indicates a Bradford Standards curve. Three unknowingly samples are taken each having two dilutions. By means of plotting graph of concentration vs absorbance at 595 nm , one can find out concentration of unknowingly taking into consideration the readings for standards.
2. This is multiplied by dilutions factors as can be seen in the table.
3. This samples graph plots the concentration value of individual sequence which shows decrease in concentration with more dilution.