Question

lab report on the detections of fats protein and carbohydrates in foods

tb ry Ogn n al G Tes REPORT SHEET Organie Punetional Group Tests A Charactertaing Known Compounds 1. Solobility Tests Indicate whicch compounds are soluble in deionized water, and for those that are soluble, indicate whether the solution is acidic, neutral or basic wwe Compeued Selubility (mark+ ar-) Addic, Besic or Neutral Aniline Propenone Diethylomine Benzok acid Which compounds are insoluble in woter but soluble in 6 M NoOH Which compounds are insoluble in weter ond 6 M NOOH but soluble in 6 M HC? Note: For tests 2 through 6, carefully record your observations. Accurately describe each positive test and include factors such as reaction time. color change, precipitate formation, and the need for heating stirring or shaking. Ceric Nitrate Test 2. Known Compound: Ethanol (ethyl alcohol) Observations 1. Define the following terms carbohydrate b. protein monosaccharide C. d. fatty acid acid hydrolysis f. triglyceride 2. Give a word equation that describes the reaction of glucose with Benedict r Laboratory 7 The Deection of Fats, Protens and Corbohydrates in Foodsu 87 QUESTIONS 1. Define the following terms carbohydrate b. protein monosaccharide d. fatty acid acid hydrolysis triglyceride 2. Give a word equation that describes the reaction of glucose with Benedict reagent. 0

Answer 1

Ans:

1) Carbohydrates: these are biomolecules, which consiitng of carbon, oxygen and hydrogen elements. These can be sugars, starches and fiber found in froots and vegetables. Primary unit in carbohydrate is CH₂O. The carbohydrates may be monosaccharides, disaccharides or poly saccharides.

    Proteins: These are very essential part in living organisms for structure and function of cells and tissues. These are naturally occuring complex compounds formed from different combination of 20 amino acids by forming amide linkage ( Peptide bond). Ex: -CO-NH- (Peptide bond)

    Monosaccharide: These are the simplest sugars in carbohydrates, which cannot be further hydrolysed. Ex: Glucose

    Fatty acids: Fatty acids, those consists carboxylic functional group with longest saturated or unsaturated aliphatic carbon chain. Ex: Fats and oils

Acid hydrolysis: If hydrolysis of carboxylic acid derivatives ( such as esters) were carried out in presence of acid (such as HCl) to get carboxylic acids, is called acid hydrolysis

Triglycerides: Triglycerides are a type of fat in human blood, which consists of ester functional group can be synthesizes from glycerol and fatty acids.

2) Benedict's reagent is a mixture of sodium carbonate, sodium citrate and copper (II) sulphate. This reagent reacts with the reducing sugars such as carbohydrates with free aldehydes and converts aldehyde group into carbaxylic acids by oxidation reaction. Reagent under go reduction, and cu (II) converted into Cu(I). So color change can be observed from blue color to brown color after reacting the Benedict's reagent with reducing sugars.