Homework Answers
Molecular weight determination of protein and its identification by mass spectrometry.
To calculate the intact protein mass through detected m/z value of two neighbouring charge states we use an equation which is explained below.
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Answer question 1.
A little more about M/Z • Mass to Charge Ratio • m/z= (mass...
6) MALDI cluster masses: Assume that your substrate M shows a peak at an m/z ratio...
6) MALDI cluster masses: Assume that your substrate M shows a peak at an m/z ratio of 201 for the [M+H]+ pseudomolecular ion. (A) Determine the m/z value for the following ions that might also be observed, then (B) sketch a mass spectrum that contains equal amounts of each of the ions (i – v), along with the pseudomolecular ion, in the m/z range 0-600. Label each peak with its ion formula. i) [M2+H]+ ii) M2+ iii) M22+ iv) M2+...
about the olgin that has Tadius < InetelS. 3.2 The surface charge density throughout the z...
about the olgin that has Tadius < InetelS. 3.2 The surface charge density throughout the z = 0 plane is ps-1/plc/m2], where p is specified in meters. Find the total charge Q that is contained in a circulai disk of radius 1.0 [m] that is centered about the origin in the same plane. 3.3 The current density in a region is J = xy [A/m2], where x and y are specified in meters. Find the current I flowing towards increasing...
A particle of charge q and mass m is bound in the ground state of a one-dimensional harmonic osci...
A particle of charge q and mass m is bound in the ground state of a one-dimensional harmonic oscillator potential with frequency oo. At time t-0 a weak spatially uniform electric field (E) is turned on, so that the perturbation to the Hamiltonian can be described as R'(t) =-q Exe-t/t for t> 0. Using first order, time-dependent perturbation theory, calculate the following probabilities: (a) the particle is detected in the first excited state after a very long time (t »...
Explain how a mass spectrometer is used to separate ions of different mass-to-charge (m/z) ratios by...
Explain how a mass spectrometer is used to separate ions of different mass-to-charge (m/z) ratios by placing the statements in the order in which they occur. First step Gaseous ions are created in the ion source. The ions enter the vacuum chamber of the mass spectrometer A magnetic field is applied to the ions. The ions are deflected by the magnetic field. The velocity of the ions is increased as they pass through metal plates with a voltage applied to...
Using the tables answer the question 1, (under questions) Mass Data and Calculations Table Aluminum Brass...
Using
the tables answer the question 1, (under questions)
Mass Data and Calculations Table Aluminum Brass Iron M1 (kg) | M2(kg) | M3 (kg) | M4 (kg) | M(kg) | ơn--1 (kg) | 때(kg) 0.0863 00864 0.086 0086 0.08631 1.436,10 .18 l0 02699 0.2699 0.26 0.260 0.2696945830 .2810 Zero Reading of the calipers Length Data and Calculations Table 0.0315 0.0314 0035 0.0349 0.00165 8.250" Aluminum 0.0318 10.0318 | 0.0310|0.0316 |0.0318 |1.633x10"18-16 SUP 00315 0.0315 lo'031S 0,0316 0,0315 ч L0824(1014.12(10 Iron...
The following system is composed by two masses The first mass m, = 21 kg, moving...
The following system is composed by two masses The first mass m, = 21 kg, moving horizontally (x1, positive rightwards) • The second mass m2 = 2.4 kg, moving horizontally (X2. positive rightwards) The first mass is connected to the ground (on the left) by two springs, each with stiffness k = 201 N/m. The second mass is connected to the first mass by another spring, also with stiffness k = 201 N/m. A harmonic force is applied to the...
1. A block of mass m = 0.23 kg slides with initial velocityv0 = 1.4...
1. A block of mass m = 0.23 kg slides with initial velocity
v0 = 1.4 m/s along a frictionless surface (as in the
figure). The block next slides through a region of length d = 0.25
m with kinetic friction. After passing through the friction region,
the block slides up a curved ramp until momentarily coming to rest
at a height h above the level surface. If the kinetic friction
coefficient μ k = 0.204, what height h does...
Finding the charge to mass (e/m) ratio of the electron Often in physics, we look for ways to find fundamental physical...
Finding the charge to mass (e/m) ratio of the electron Often in physics, we look for ways to find fundamental physical constants. From the set-up demonstrated in class, we are able to find a monumental combination of two physical constants-the charge of an electron (e) and the mass of an electron (m). Since the charge of an electron (e) is well known through other experiments, we will use the ratio of charge to mass to find the mass of an...
Finding the charge to mass (e/m) ratio of the electron Often in physics, we look for ways to find fundamental physical...
Finding the charge to mass (e/m) ratio of the electron Often in physics, we look for ways to find fundamental physical constants. From the set-up demonstrated in class, we are able to find a monumental combination of two physical constants-the charge of an electron (e) and the mass of an electron (m). Since the charge of an electron (e) is well known through other experiments, we will use the ratio of charge to mass to find the mass of an...
Derive the equation e/m = (3.29x106)*(Va)/(I2)(R2) for the charge to mass ratio of an electron using...
Derive the equation e/m = (3.29x106)*(Va)/(I2)(R2) for the charge to mass ratio of an electron using the following equations: (1): (e)(Va) = 1/2 mv2 (2): R = mv/eB (3): B = ((4/5)3/2 ) * (µ0)(N)(I)/r where µ0 = 4π × 10−7 H/m ; N = 130; and r = 0.15m
6) MALDI cluster masses: Assume that your substrate M shows a peak at an m/z ratio of 201 for the [M+H]+ pseudomolecular ion. (A) Determine the m/z value for the following ions that might also be observed, then (B) sketch a mass spectrum that contains equal amounts of each of the ions (i – v), along with the pseudomolecular ion, in the m/z range 0-600. Label each peak with its ion formula. i) [M2+H]+ ii) M2+ iii) M22+ iv) M2+...
about the olgin that has Tadius < InetelS. 3.2 The surface charge density throughout the z = 0 plane is ps-1/plc/m2], where p is specified in meters. Find the total charge Q that is contained in a circulai disk of radius 1.0 [m] that is centered about the origin in the same plane. 3.3 The current density in a region is J = xy [A/m2], where x and y are specified in meters. Find the current I flowing towards increasing...
A particle of charge q and mass m is bound in the ground state of a one-dimensional harmonic oscillator potential with frequency oo. At time t-0 a weak spatially uniform electric field (E) is turned on, so that the perturbation to the Hamiltonian can be described as R'(t) =-q Exe-t/t for t> 0. Using first order, time-dependent perturbation theory, calculate the following probabilities: (a) the particle is detected in the first excited state after a very long time (t »...
Explain how a mass spectrometer is used to separate ions of different mass-to-charge (m/z) ratios by placing the statements in the order in which they occur. First step Gaseous ions are created in the ion source. The ions enter the vacuum chamber of the mass spectrometer A magnetic field is applied to the ions. The ions are deflected by the magnetic field. The velocity of the ions is increased as they pass through metal plates with a voltage applied to...
Using
the tables answer the question 1, (under questions)
Mass Data and Calculations Table Aluminum Brass Iron M1 (kg) | M2(kg) | M3 (kg) | M4 (kg) | M(kg) | ơn--1 (kg) | 때(kg) 0.0863 00864 0.086 0086 0.08631 1.436,10 .18 l0 02699 0.2699 0.26 0.260 0.2696945830 .2810 Zero Reading of the calipers Length Data and Calculations Table 0.0315 0.0314 0035 0.0349 0.00165 8.250" Aluminum 0.0318 10.0318 | 0.0310|0.0316 |0.0318 |1.633x10"18-16 SUP 00315 0.0315 lo'031S 0,0316 0,0315 ч L0824(1014.12(10 Iron...
The following system is composed by two masses The first mass m, = 21 kg, moving horizontally (x1, positive rightwards) • The second mass m2 = 2.4 kg, moving horizontally (X2. positive rightwards) The first mass is connected to the ground (on the left) by two springs, each with stiffness k = 201 N/m. The second mass is connected to the first mass by another spring, also with stiffness k = 201 N/m. A harmonic force is applied to the...
1. A block of mass m = 0.23 kg slides with initial velocity
v0 = 1.4 m/s along a frictionless surface (as in the
figure). The block next slides through a region of length d = 0.25
m with kinetic friction. After passing through the friction region,
the block slides up a curved ramp until momentarily coming to rest
at a height h above the level surface. If the kinetic friction
coefficient μ k = 0.204, what height h does...
Finding the charge to mass (e/m) ratio of the electron Often in physics, we look for ways to find fundamental physical constants. From the set-up demonstrated in class, we are able to find a monumental combination of two physical constants-the charge of an electron (e) and the mass of an electron (m). Since the charge of an electron (e) is well known through other experiments, we will use the ratio of charge to mass to find the mass of an...
Finding the charge to mass (e/m) ratio of the electron Often in physics, we look for ways to find fundamental physical constants. From the set-up demonstrated in class, we are able to find a monumental combination of two physical constants-the charge of an electron (e) and the mass of an electron (m). Since the charge of an electron (e) is well known through other experiments, we will use the ratio of charge to mass to find the mass of an...
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