1.Name the reagent antigen in the ANA test. 2.What is the composition of the conjugate? 3.What...
1.Name the reagent antigen in the ANA test.
2.What is the composition of the conjugate?
3.What is the substrate for this test?
4.What does the negative control look like?
5.Which ANA pattern is highly suggestive of SLE?
Why?
Homework Answers
1. Anti human immunoglobulin
2.FITC labelled anti human igG
3. Human epithelial type-2 (hep2)
4 sample consider negative for Ana if 1+Flouroscent at serum dilution of 1:40
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1.Name the reagent antigen in the ANA test.
2.What is the composition of the conjugate?
3.What...
1. What does an ELISA test for? 2. What variables can affect the outcome? (Name 4)...
1. What does an ELISA test for? 2. What variables can affect the outcome? (Name 4) 3. What are the three important limitations of an ELISA? Explain each. 4. In the virtual lab, the ELISA plate has been pretreated to bind SLE antigen to each well. WHY? How do you expect the assay setup to differ in our case where the antigen of interest is present in a heterogenous sample in small quantities? 5. Why are there both a positive...
pls help, ty ELISA Enzyme-Linked Immunosorbant Assay ELISA is a rapid test used for detecting or...
pls help, ty
ELISA Enzyme-Linked Immunosorbant Assay ELISA is a rapid test used for detecting or quantifying antibody (Ab) binding against viruses, bacteria and other materials or antigens (Ag). Can detect antibody antigen interaction. 96-well plates are made of polystyrene and are coated with either antigen or antibody. The function of the plate has to hold the immobilized either antigen or antibody. Legend for diagram below: Ag = antigen E = enzyme Substrate interacts with enzyme to allow colorimetric detection...
Microbiology ONLY answe 1 2 3 4 5 6 Which reagent is added to test for...
Microbiology
ONLY answe
1
2
3
4
5
6
Which reagent is added to test for indole? A“positive” lactose test is yellow. What is present in the medium that causes the color change? You added methyl red to your MR test and observe a red color. The test result can be interpreted as You forgot to label a tube with a slant but you remember that its original color was green and now after incubation, the slant color is blue....
please help me out! Negative and positive controls Tube 1: 1 ml Catalase + 3 ml...
please help me out!
Negative and positive controls Tube 1: 1 ml Catalase + 3 ml distilled water Tube 2: 1 ml Catalase + 3 ml sucrose Tube 3: 1 ml Catalase + 3 ml H,O, Positive test produces bubbles; negative reaction does not bubble Question #1: State the NUMBER of the ONE tube that is the negative control: Question #2: Explain why no bubbles will appear in Tube 2: Effect of concentration - Tube 4: 1 ml catalase +...
QUESTION 1 What is a heterophile antigen? a. an antigen homospecific to only one antibody. b....
QUESTION 1 What is a heterophile antigen? a. an antigen homospecific to only one antibody. b. an antigen that has been modified to bind to more than one antibody. c. an antigen found only on one etiological agent. d. an antigen common to more than one species and whose distribution is unrelated to its phylogenetic distribution. 1 points QUESTION 2 What is an antigen? a protein synthesized and secreted by T lymphocytes (T cells). All bacterial cells are antigens....
aeruginosa 4. Which families of organisms are differentiated by the Oxidase test? What reagent is used...
aeruginosa 4. Which families of organisms are differentiated by the Oxidase test? What reagent is used to test for Oxidase? 5. Which families of organisms are differentiated by the Catalase test? What is a positive test for catalase? 6. What substrate is used to test for the presence of the Coagulase enzyme? What does Coagulase do for a bacteria that has it?
Exercise 41 А Photos 1. What is the name of this test? 2. Which test is...
Exercise 41 А Photos 1. What is the name of this test? 2. Which test is positive? 3. Which test is negative? 4. What enzyme is being tested for with this test? 5. What is the function of the enzyme being tested for? I
Questions: 1. What is responsible for a positive Benedict's test? 12-KI test? 2. What is responsi...
Questions: 1. What is responsible for a positive Benedict's test? 12-KI test? 2. What is responsible for a positive Biuret test? How are lipids detected? 3. Which biomolecules are present in the food samples? Procedure: Benedict's test 1. Fill 100 mL Beaker with 40 mL of water. 2. Place the burner stand over the burner and light it up using a match. 3. Place the beaker containing water over the burner stand to make a water bath. 4. Label 3...
369 Name Locker Number Date Plesse print: last name first REPORT SHEET (continued): Experiment 26 Hydrolysis of a D...
369 Name Locker Number Date Plesse print: last name first REPORT SHEET (continued): Experiment 26 Hydrolysis of a Disaccharide 5. Does sucrose give a positive Benedict test? Why or why not? After hydrolysis, did sucrose give a positive Benedict test? Explain your answer. 6. Bial Test What color indicates a positive test for a pentose sugar? Record your observations +or - for each carbohydrate in the table below: 1. xylose 3. fructose 5. starch 2. glucose 6. control 4. sucrose...
Exercise 40: I A B B 1. What is the name of this test? 2. Which...
Exercise 40: I A B B 1. What is the name of this test? 2. Which test is positive? 3. Which test is negative? 4. What enzymes are being tested for with this test? 5. What is the function of the enzymes being tested for?
pls help, ty
ELISA Enzyme-Linked Immunosorbant Assay ELISA is a rapid test used for detecting or quantifying antibody (Ab) binding against viruses, bacteria and other materials or antigens (Ag). Can detect antibody antigen interaction. 96-well plates are made of polystyrene and are coated with either antigen or antibody. The function of the plate has to hold the immobilized either antigen or antibody. Legend for diagram below: Ag = antigen E = enzyme Substrate interacts with enzyme to allow colorimetric detection...
Microbiology
ONLY answe
1
2
3
4
5
6
Which reagent is added to test for indole? A“positive” lactose test is yellow. What is present in the medium that causes the color change? You added methyl red to your MR test and observe a red color. The test result can be interpreted as You forgot to label a tube with a slant but you remember that its original color was green and now after incubation, the slant color is blue....
please help me out!
Negative and positive controls Tube 1: 1 ml Catalase + 3 ml distilled water Tube 2: 1 ml Catalase + 3 ml sucrose Tube 3: 1 ml Catalase + 3 ml H,O, Positive test produces bubbles; negative reaction does not bubble Question #1: State the NUMBER of the ONE tube that is the negative control: Question #2: Explain why no bubbles will appear in Tube 2: Effect of concentration - Tube 4: 1 ml catalase +...
aeruginosa 4. Which families of organisms are differentiated by the Oxidase test? What reagent is used to test for Oxidase? 5. Which families of organisms are differentiated by the Catalase test? What is a positive test for catalase? 6. What substrate is used to test for the presence of the Coagulase enzyme? What does Coagulase do for a bacteria that has it?
Exercise 41 А Photos 1. What is the name of this test? 2. Which test is positive? 3. Which test is negative? 4. What enzyme is being tested for with this test? 5. What is the function of the enzyme being tested for? I
Questions: 1. What is responsible for a positive Benedict's test? 12-KI test? 2. What is responsible for a positive Biuret test? How are lipids detected? 3. Which biomolecules are present in the food samples? Procedure: Benedict's test 1. Fill 100 mL Beaker with 40 mL of water. 2. Place the burner stand over the burner and light it up using a match. 3. Place the beaker containing water over the burner stand to make a water bath. 4. Label 3...
369 Name Locker Number Date Plesse print: last name first REPORT SHEET (continued): Experiment 26 Hydrolysis of a Disaccharide 5. Does sucrose give a positive Benedict test? Why or why not? After hydrolysis, did sucrose give a positive Benedict test? Explain your answer. 6. Bial Test What color indicates a positive test for a pentose sugar? Record your observations +or - for each carbohydrate in the table below: 1. xylose 3. fructose 5. starch 2. glucose 6. control 4. sucrose...
Exercise 40: I A B B 1. What is the name of this test? 2. Which test is positive? 3. Which test is negative? 4. What enzymes are being tested for with this test? 5. What is the function of the enzymes being tested for?
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