Question

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ELISA Enzyme-Linked Immunosorbant Assay ELISA is a rapid test used for detecting or quantifying antibody (Ab) binding against viruses, bacteria and other materials or antigens (Ag). Can detect antibody antigen interaction. 96-well plates are made of polystyrene and are coated with either antigen or antibody. The function of the plate has to hold the immobilized either antigen or antibody.

Answer 1

Answer -le other less Direct ELISA detection is faster than types because in this technique there is steps & fewer reagents are needed which no potentially cross- reacting condary antibody needed by secondary antibody needed. Answer - 2 e senstivity Secondary The Indirect ELISA since more than antibody can bind has high one labeled the primary antibody Answer- Chromogen Istobostrate - bonded with enzyme - labeled antiboag enzyme Autigen. Captured cute body. authbody o capture Ab - Ag added to Sample - Labelled Ab Ts added substrate & Thromophore is added controls needs . Answer-t → There is fallowing fallowing 2) Postivie control a) Negative control het i V Standard Pro standard in sample matory canne endogenous positive control LA