Question

ELISA-HIV Testing DATA SHEET RESULTS For each of the following, report the color and indicate whether the result was positive or negative. Neg.control: negative Donorl: negative Pos. control: positive Donor 2: positive Based on your results, which donor has been exposed to HIV? Donor 2 QUESTIONS 1 Describe the purpose of the two controls. The purpose of the two controls is to show what the positive and negative would look like, and it also helps determine it the experiment was done correctly 2 a. If performing this ELISA test and one of the wells for Donor I was pink and the other was colorless, what would be a possible explanation? b. What should be done with this result for Donor 1? 3 Is this test detecting AIDS or HIV infection? Explain your answer. This test is detecting the present of antibodies that fight off HIV, The bottom of the plate is coated with the HIV antigen, so positive results means HIV antibodies are present.

Answer 1

1. The negative controller is used to show that any positive effects of the new treatment aren't the result of of the placebo effect.The positive control is used to detect any problems with the experiment.

4. If a patient's ELISA test is positive,then more sophisticated tests are done to confirm whether the person has HIV. These tests include the differentiation assay and a test called the nucleic acid test(NAT).

5. The correct order for an indirect ELISA test are: known antigen,patient serum,enzyme lined 2⁰Ab,substrate

6.The secondary antibodies bind to the primary antibody to assist in detection,sorting and purification of target antigens.

8. In a direct ELISA we are detecting the presence of an antigen and the primary antibody used is the enzyme linked antibody.In an indirect ELISA we are detecting the antibody.