Plot the titration curve for Bicine and calculate how to prepare a pH 7.5 Bicine buffer so...

Plot the titration curve for Bicine and calculate how to prepare a pH 7.5 Bicine buffer solution Bicine (N, N–bis (2-hydroxyethyl) glycine) is another commonly used buffer in biochemistry labs. The structure of bicine in its fully protonated form is shown below:

a. Draw the titration curve for Bicine, assuming the pKa for its free COOH group is 2.3 and the pKa for its tertiary amino group is 8.3.

---

b. Draw the structure of the fully deprotonated form (completely dissociated form) of bicine.

---

c. You have available a 0.1 M solution of Bicine at its isoelectric point (pHI), 0.1 M solutions of HCl and NaOH, and ample distilled H2O. Describe the preparation of 1 L of 0.04 M Bicine buffer, pH 7.5.

---

d. What is the concentration of fully protonated form of Bicine in your final buffer solution?