How to prepare a buffer solution: a Tris buffer solutionTris-hydroxymethyl aminomethane (T...

How to prepare a buffer solution: a Tris buffer solution

Tris-hydroxymethyl aminomethane (TRIS) is widely used for the preparation of buffers in biochemical

research. Shown here is the structure TRIS in its protonated form:

Its acid dissociation constant, Ka, is 8.32 x 10-9. You have available at your lab bench a 0.1 M solution of TRIS in its protonated form, 0.1 M solutions of HCl and NaOH, and ample distilled water. Describe the preparation of a 1 L solution of 0.02 M TRIS buffer, pH 7.8.