Adapted from M. L. Shuler and F. Kargi, Bioprocess Engineering, Prentice Hall (2002). Cell...

Adapted from M. L. Shuler and F. Kargi, Bioprocess Engineering, Prentice Hall (2002). Cell growth takes place in bioreactors called chemostats.

A substrate such as glucose is used to grow cells and produce a product.

A generic molecule formula for the biomass is C4.4H7.3N0.86O1.2. Consider the growth of a generic organism on glucose

Experimentally, it was shown that for this organism, the cells convert 2/3 of carbon substrate to biomass.

(a) Calculate the stoichiometric coefficients a, b, c, d, and e (Hint: carry out atom balances [Ans: c = 0.91]).

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(b) Calculate the yield coefficients YC/S (g cells/g substrate) and YC/O2 (g cells/g O2). The gram of cells are dry weight (no water—gdw = (gdw = grams dry weight).